Literature summary for 2.1.1.228 extracted from

Li, Y.; Zhong, W.; Koay, A.Z.; Ng, H.Q.; Nah, Q.; Wong, Y.H.; Hill, J.; Lescar, J.; Dedon, P.C.; Kang, C.
Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa (2019), Biomol. NMR Assign., 13, 327-332 .

Search for more literature for 2.1.1.228

Application

Application	Comment	Organism
drug development	bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) from Pseudomonas aeruginosa is a good target for development of antibacterial compounds	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
gene trmD, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
sinefungin	competitive inhibitor	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa UCBPP-PA14	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q02RL6	-	-
Pseudomonas aeruginosa UCBPP-PA14	Q02RL6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration	Pseudomonas aeruginosa

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Subunits

Subunits	Comment	Organism
dimer	enzyme TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-L-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer	Pseudomonas aeruginosa
More	backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
TrmD	-	Pseudomonas aeruginosa
tRNA-(N1G37) methyltransferase	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
additional information	backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis	Pseudomonas aeruginosa
physiological function	methylation of guanine at position 37 in RNA is critical for bacterial growth, as this reaction is essential for preventing reading frame shifts during translation and therefore for maintaining the fidelity of protein synthesis. This methylation is catalyzed by the tRNA (guanine37-N1)-methyltransferase (TrmD). TrmD uses S-adenosyl-l-methionine (SAM) as a cofactor and transfers the methyl group to the N1 atom of G37 in tRNA. Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation. and is critical for growth of Pseudomonas aeruginosa	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)