Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.228 extracted from

  • Paris, Z.; Horakova, E.; Rubio, M.A.; Sample, P.; Fleming, I.M.; Armocida, S.; Lukes, J.; Alfonzo, J.D.
    The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function (2013), RNA, 19, 649-658.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene trm5, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information generation of enzyme knockout lines using a TbTRM5 RNAi plasmid in procyclic Trypanosoma brucei 29-13 cells Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion TbTRM5 localizes to nucleus and mitochondrion Trypanosoma brucei brucei 5739
-
nucleus TbTRM5 localizes to nucleus and mitochondrion Trypanosoma brucei brucei 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU Trypanosoma brucei brucei TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU Trametes pubescens 927 / 4 GUTat10.1 / TREU927 TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?

Organism

Organism UniProt Comment Textmining
Trametes pubescens 927 / 4 GUTat10.1 / TREU927 Q57X10
-
-
Trypanosoma brucei brucei Q57X10
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in tRNAArgCCG
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArgCCG
-
?
S-adenosyl-L-methionine + guanine37 in tRNAArgCCG
-
Trametes pubescens 927 / 4 GUTat10.1 / TREU927 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAArgCCG
-
?
S-adenosyl-L-methionine + guanine37 in tRNACysGCA
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACysGCA
-
?
S-adenosyl-L-methionine + guanine37 in tRNACysGCA
-
Trametes pubescens 927 / 4 GUTat10.1 / TREU927 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACysGCA
-
?
S-adenosyl-L-methionine + guanine37 in tRNAHisGUG
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHisGUG
-
?
S-adenosyl-L-methionine + guanine37 in tRNAHisGUG
-
Trametes pubescens 927 / 4 GUTat10.1 / TREU927 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAHisGUG
-
?
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37 Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU
-
Trametes pubescens 927 / 4 GUTat10.1 / TREU927 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?
S-adenosyl-L-methionine + guanine37 in tRNAIleUaU TbTRM5 is responsible for m1G37 formation in several tRNAs, cytosolic tRNAIle UAU is essentially fully modified at G37 Trametes pubescens 927 / 4 GUTat10.1 / TREU927 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAIleUaU
-
?
S-adenosyl-L-methionine + guanine37 in tRNALeuCAG
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeuCAG
-
?
S-adenosyl-L-methionine + guanine37 in tRNAProAGG
-
Trypanosoma brucei brucei S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAProAGG
-
?

Synonyms

Synonyms Comment Organism
TRM5
-
Trypanosoma brucei brucei

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Trypanosoma brucei brucei

General Information

General Information Comment Organism
malfunction downregulation of TRM5 by RNAi leads to the expected disappearance of m1G37, but with little effect on cytoplasmic translation. Lack of m1G37 does not globally affect cytosolic translation. On the contrary, lack of TRM5 causes a marked growth phenotype and a significant decrease in mitochondrial functions, including protein synthesis Trypanosoma brucei brucei
physiological function TRM5 is responsible for m1G37 formation, m1G37 formation in mitochondria is important for respiration, and TbTRM5 is important for mitochondrial protein synthesis and biogenesis. Mitochondrial TRM5 may be needed to mature unmethylated tRNAs that reach the mitochondria and that can pose a problem for translational fidelity, lack of import specificity between some fully matured and potentially defective tRNA species Trypanosoma brucei brucei