KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Escherichia coli | |
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Methanocaldococcus jannaschii | |
0.0012 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 52°C | Methanocaldococcus jannaschii | |
0.0028 | - |
guanine37 in Escherichia coli tRNAPro | pH 8.0, 37°C | Escherichia coli | |
0.0073 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | Q58293 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNAPro | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNAPro | - |
? | |
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro | - |
? | |
S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRM5 | - |
Methanocaldococcus jannaschii |
TrmD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assas at | Escherichia coli |
52 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Escherichia coli | |
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Methanocaldococcus jannaschii | |
0.0086 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 52°C | Methanocaldococcus jannaschii | |
0.01 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 37°C | Escherichia coli | |
0.017 | - |
guanine37 in Escherichia coli tRNAPro | pH 8.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | the m1G37 modification prevents tRNA frameshifts on the ribosome by assuring correct codon-anticodon pairings, and thus is essential for the fidelity of protein synthesis | Escherichia coli |
physiological function | the m1G37 modification prevents tRNA frameshifts on the ribosome by assuring correct codon-anticodon pairings, and thus is essential for the fidelity of protein synthesis | Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Escherichia coli | |
additional information | - |
additional information | kinetic analysis of tRNA truncation mutants and tRNA mutant with alterations in the anticodon loop reveals that TrmD and Trm5 exhibit separate and distinct mode of tRNA recognition, suggesting that they evolved by independent and nonoverlapping pathways from their unrelated AdoMet families | Methanocaldococcus jannaschii | |
1.7 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 37°C | Escherichia coli | |
6.1 | - |
guanine37 in Escherichia coli tRNAPro | pH 8.0, 37°C | Escherichia coli | |
7.22 | - |
guanine37 in Methanocaldococcus jannaschii tRNAPro | pH 8.0, 52°C | Methanocaldococcus jannaschii |