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Literature summary for 2.1.1.227 extracted from
- Capreomycin susceptibility is increased by TlyA-directed 2-O-methylation on both ribosomal subunits (2012), Mol. Microbiol., 85, 1194-1203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1 | Geobacillus stearothermophilus |
| gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1 | Mycobacterium tuberculosis |
| gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1 | Streptomyces coelicolor |
| gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1 | Mycolicibacterium smegmatis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Geobacillus stearothermophilus |  |
| Mg2+ | required | Mycobacterium tuberculosis | |
| Mg2+ | required | Streptomyces coelicolor | |
| Mg2+ | required | Mycolicibacterium smegmatis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | Geobacillus stearothermophilus | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | Mycobacterium tuberculosis | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | Streptomyces coelicolor | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | Mycobacterium tuberculosis H37Rv | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
gene tlyA | - |
| Mycobacterium tuberculosis | - |
gene tlyA | - |
| Mycobacterium tuberculosis H37Rv | - |
gene tlyA | - |
| Mycolicibacterium smegmatis | A0QYR0 | gene tlyA | - |
| Mycolicibacterium smegmatis mc(2)155 | A0QYR0 | gene tlyA | - |
| no activity in Escherichia coli | - |
- |
- |
| Streptomyces coelicolor | - |
gene tlyA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the TlyAII modifies C1409 in Escherichia coli 30S subunits, and activity at this site on 16S rRNA and 70S ribosomes is negligible | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | the TlyAII modifies C1409 in Escherichia coli 30S subunits, and activity at this site on 16S rRNA and 70S ribosomes is negligible | Mycolicibacterium smegmatis mc(2)155 | ? | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | - |
Geobacillus stearothermophilus | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | - |
Mycobacterium tuberculosis | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | - |
Streptomyces coelicolor | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 16S rRNA | - |
Mycobacterium tuberculosis H37Rv | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 30S rRNA | - |
Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 30S rRNA | - |
? | |
| S-adenosyl-L-methionine + cytidine1409 in 30S rRNA | - |
Mycolicibacterium smegmatis mc(2)155 | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 30S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TlyA | - |
Geobacillus stearothermophilus |
| TlyA | - |
Mycobacterium tuberculosis |
| TlyA | - |
Streptomyces coelicolor |
| TlyA | - |
Mycolicibacterium smegmatis |
| TlyAII | - |
Geobacillus stearothermophilus |
| TlyAII | - |
Mycobacterium tuberculosis |
| TlyAII | - |
Streptomyces coelicolor |
| TlyAII | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at with Mycobacterium smegmatis ribosomal substrates | Geobacillus stearothermophilus |
| 37 | - |
assay at with Mycobacterium smegmatis ribosomal substrates | Mycobacterium tuberculosis |
| 37 | - |
assay at with Mycobacterium smegmatis ribosomal substrates | Streptomyces coelicolor |
| 37 | - |
assay at with Mycobacterium smegmatis ribosomal substrates | Mycolicibacterium smegmatis |
| 50 | - |
assay at with Thermus thermophilus ribosomal substrates | Geobacillus stearothermophilus |
| 50 | - |
assay at with Thermus thermophilus ribosomal substrates | Mycobacterium tuberculosis |
| 50 | - |
assay at with Thermus thermophilus ribosomal substrates | Streptomyces coelicolor |
| 50 | - |
assay at with Thermus thermophilus ribosomal substrates | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Geobacillus stearothermophilus |
| 7.5 | - |
assay at | Mycobacterium tuberculosis |
| 7.5 | - |
assay at | Streptomyces coelicolor |
| 7.5 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Geobacillus stearothermophilus | |
| S-adenosyl-L-methionine | - |
Mycobacterium tuberculosis | |
| S-adenosyl-L-methionine | - |
Streptomyces coelicolor | |
| S-adenosyl-L-methionine | - |
Mycolicibacterium smegmatis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 and C1920 (c.f. EC 2.1.1.226) | Geobacillus stearothermophilus |
| evolution | TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 and C1920 (c.f. EC 2.1.1.226) | Mycobacterium tuberculosis |
| evolution | TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 and C1920 (c.f. EC 2.1.1.226) | Streptomyces coelicolor |
| evolution | TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 and C1920 (c.f. EC 2.1.1.226) | Mycolicibacterium smegmatis |
| malfunction | inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin | Geobacillus stearothermophilus |
| malfunction | inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin | Mycobacterium tuberculosis |
| malfunction | inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin | Streptomyces coelicolor |
| malfunction | inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin | Mycolicibacterium smegmatis |
| additional information | the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA | Geobacillus stearothermophilus |
| additional information | the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA | Mycobacterium tuberculosis |
| additional information | the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA | Streptomyces coelicolor |
| additional information | the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA | Mycolicibacterium smegmatis |
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