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Literature summary for 2.1.1.227 extracted from

  • Johansen, S.K.; Maus, C.E.; Plikaytis, B.B.; Douthwaite, S.
    Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs (2006), Mol. Cell, 23, 173-182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant tlyA in Escherichia coli markedly increases susceptibility to the drugs capreomycin and viomycin Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytidine1409 in 16S rRNA Mycobacterium tuberculosis
-
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytidine1409 in 16S rRNA
-
Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytidine1409 in 16S rRNA the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA the exact position of the methyl group in the wild-type rRNAs is verified by MALDI quadrupole-TOF tandem MS analysis ?

Synonyms

Synonyms Comment Organism
TlyA
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction the bifunctional enzyme modifies nucleotide C1409 in helix 44 of 16S rRNA and nucleotide C1920 in helix 69 of 23S rRNA. Loss of these rRNA methylations confers resistance to capreomycin and viomycin Mycobacterium tuberculosis