BRENDA - Enzyme Database show
show all sequences of 2.1.1.225

The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4

Wilkinson, M.L.; Crary, S.M.; Jackman. J.E.; Grayhack, E.J.; Phizicky, E.M.; RNA 13, 404-413 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00001
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenosine4 in tRNAHis
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
Q12383
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
(3)
Saccharomyces cerevisiae
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
(2)
Saccharomyces cerevisiae
S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
(1)
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenosine4 in tRNAHis
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + adenosine4 in tRNAHis
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.004
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00001
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenosine4 in tRNAHis
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
Saccharomyces cerevisiae
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenosine4 in tRNAHis
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + adenosine4 in tRNAHis
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
-
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
713500
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.004
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Saccharomyces cerevisiae
General Information
General Information
Commentary
Organism
malfunction
trm13-DELTA strains do not have an obvious growth defect
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
trm13-DELTA strains do not have an obvious growth defect
Saccharomyces cerevisiae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
400
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
400
-
adenosine4 in tRNAHis
pH 8.0, 30°C
Saccharomyces cerevisiae
Other publictions for EC 2.1.1.225
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712803
Tkaczuk
Trm13p, the tRNA:Xm4 modificat ...
Saccharomyces cerevisiae
J. Mol. Model.
16
599-606
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713500
Wilkinson
The 2'-O-methyltransferase res ...
Saccharomyces cerevisiae
RNA
13
404-413
2007
-
-
1
-
-
-
-
1
-
-
-
3
-
5
-
-
1
3
-
-
-
-
6
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
1
-
-
-
-
6
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1