Literature summary for 2.1.1.224 extracted from

Yan, F.; Fujimori, D.G.
RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift (2011), Proc. Natl. Acad. Sci. USA, 108, 3930-3934.

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Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA	the newly introduced methyl group is assembled from an S-adenosyl-L-methionine (SAM)-derived methylene fragment and a hydrogen atom that had migrated from the substrate amidine carbon. Rather than activating the adenosine nucleotide of the substrate by hydrogen atom abstraction from an amidine carbon, the 5'-deoxyadenosyl radical abstracts hydrogen from the second equivalent of SAM to form the SAM-derived radical cation. This species, or its corresponding sulfur ylide, subsequently adds into the substrate, initiating hydride shift and S-adenosylhomocysteine elimination to complete the formation of the methyl group	Escherichia coli	S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
Cfr	-	Escherichia coli

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Release 2023.1 (January 2023)