BRENDA - Enzyme Database show
show all sequences of 2.1.1.222

Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli

Xing, L.; Zhu, Y.; Fang, P.; Wang, J.; Zeng, F.; Li, X.; Teng, M.; Li, X.; Acta Crystallogr. Sect. F 67, 727-729 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5 and 20% (w/v) polyethylene glycol 10000 or 10% (v/v) 2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.0
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
L132Q
the mutation results in ubiquinone deficiency in Escherichia coli leading to a high sensitivity to thiols and a failure to grow on succinate
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26600
-
x * 26600, SDS-PAGE
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography, Superdex 200 gel filtration, and DEAE anion-exchange chromatography
Escherichia coli
Subunits
Subunits
Commentary
Organism
?
x * 26600, SDS-PAGE
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5 and 20% (w/v) polyethylene glycol 10000 or 10% (v/v) 2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.0
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L132Q
the mutation results in ubiquinone deficiency in Escherichia coli leading to a high sensitivity to thiols and a failure to grow on succinate
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26600
-
x * 26600, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography, Superdex 200 gel filtration, and DEAE anion-exchange chromatography
Escherichia coli
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 26600, SDS-PAGE
Escherichia coli
General Information
General Information
Commentary
Organism
metabolism
the enzyme is required for both O-methylation steps in ubiquinone biosynthesis
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is required for both O-methylation steps in ubiquinone biosynthesis
Escherichia coli
Other publictions for EC 2.1.1.222
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733306
Zhu
Structural and biochemical stu ...
Escherichia coli
Biochem. J.
470
105-114
2015
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1
1
8
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734623
Lu
Identification and elimination ...
Rhodobacter sphaeroides
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208-216
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1
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1
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1
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733281
Lu
Enhanced production of CoQ10 b ...
Rhodobacter sphaeroides
Biochem. Eng. J.
72
42-47
2013
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1
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1
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1
1
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718512
Xing
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. F
67
727-729
2011
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1
1
1
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1
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1
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1
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1
1
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718535
Gonidakis
Lifespan extension and paraqua ...
Escherichia coli, Escherichia coli BW25113
Aging
3
291-303
2011
-
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1
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28
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704270
Joyce
Reed, J.L.; White, A.; Edwards ...
Escherichia coli
J. Bacteriol.
188
8259-8271
2006
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1
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1
1
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485141
Jonassen
Isolation and functional expre ...
Homo sapiens
J. Biol. Chem.
275
12381-12387
2000
-
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1
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1
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1
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1
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1
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1
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1
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3
1
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705549
Soballe
Ubiquinone limits oxidative st ...
Escherichia coli
Microbiology
146
787-796
2000
-
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1
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1
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485140
Poon
Yeast and rat Coq3 and Escheri ...
Escherichia coli, Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
274
21665-21672
1999
-
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7
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3
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702193
Hsu
Complementation of coq3 mutant ...
Escherichia coli
Biochemistry
35
9797-9806
1996
-
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1
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1
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1
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704242
Gibert
Regulation of ubiG gene expres ...
Escherichia coli
J. Bacteriol.
170
1346-1349
1988
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