Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5 and 20% (w/v) polyethylene glycol 10000 or 10% (v/v) 2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.0 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L132Q | the mutation results in ubiquinone deficiency in Escherichia coli leading to a high sensitivity to thiols and a failure to grow on succinate | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26600 | - |
x * 26600, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Superdex 200 gel filtration, and DEAE anion-exchange chromatography | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
? | x * 26600, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UbiG | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is required for both O-methylation steps in ubiquinone biosynthesis | Escherichia coli |