Cloned (Comment) | Organism |
---|---|
gene trm10, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli | Saccharomyces cerevisiae |
gene trm10, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Saccharomyces cerevisiae | |
additional information | - |
additional information | steady-state kinetics, overview | Homo sapiens | |
0.00016 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens | |
0.00023 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens | |
0.00186 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae | |
0.0024 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | substrate specificity analysis in vivo, overview | ? | - |
? | |
additional information | Saccharomyces cerevisiae | there are 19 unique tRNA species that contain a G at position 9 in yeast, and whose fully modified sequence is known, only 9 of these tRNA species are modified with m1G9 in wild-type cells. The in vitro methylation activity of yeast Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity, substrate specificity analysis in vivo, overview | ? | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | Homo sapiens | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNACysGCA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNACysGCA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyCCC | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyCCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyGCC | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyGCC | Homo sapiens | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALeuCAA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALeuGAG | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALeuGAG | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALysCUU | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALysCUU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAThrAGU | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAThrAGU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAThrCGU | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAThrCGU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAValUAC | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAValUAC | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TBZ6 | - |
- |
Saccharomyces cerevisiae | Q12400 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Saccharomyces cerevisiae |
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity analysis in vivo, overview | Homo sapiens | ? | - |
? | |
additional information | there are 19 unique tRNA species that contain a G at position 9 in yeast, and whose fully modified sequence is known, only 9 of these tRNA species are modified with m1G9 in wild-type cells. The in vitro methylation activity of yeast Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity, substrate specificity analysis in vivo, overview | Saccharomyces cerevisiae | ? | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNACysGCA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNACysGCA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyCCC | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyCCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyGCC | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAGlyGCC | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAGlyGCC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALeuCAA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALeuCAA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALeuGAG | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALeuGAG | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNALysCUU | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNALysCUU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAThrAGU | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAThrAGU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAThrCGU | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAThrCGU | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAValUAC | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAValUAC | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNAValUAC | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNAValUAC | - |
? |
Synonyms | Comment | Organism |
---|---|---|
m1G9 methyltransferase | - |
Saccharomyces cerevisiae |
m1G9 methyltransferase | - |
Homo sapiens |
Trm10 | - |
Saccharomyces cerevisiae |
Trm10 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens | |
0.0052 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae | |
0.009 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae | |
0.012 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | |
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | N-1 methylation of the nearly invariant purine residue found at position 9 of tRNA is a nucleotide modification found in multiple tRNA species throughout Eukarya and Archaea | Homo sapiens |
evolution | N-1 methylation of the nearly invariant purine residue found at position 9 of tRNA is a nucleotide modification found in multiple tRNA species throughout Eukarya and Archaea. The tRNA methyltransferase Trm10 is a highly conserved protein both necessary and sufficient to catalyze all known instances of m1G9 modification in yeast | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae | |
3.75 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Saccharomyces cerevisiae | |
52.17 | - |
guanine9 in tRNAValUAC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens | |
75 | - |
guanine9 in tRNAGlyGCC | pH 8.0, 30°C, recombinant enzyme | Homo sapiens |