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Literature summary for 2.1.1.220 extracted from

  • Saikia, M.; Fu, Y.; Pavon-Eternod, M.; He, C.; Pan, T.
    Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs (2010), RNA, 16, 1317-1327.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.220

Protein Variants

Protein Variants Comment Organism
additional information siRNA knockdown of m1A58 methyltransferase subunits TRM6 or TRM61 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in tRNA Homo sapiens modification at the conserved nucleotide 58 in the TCC loop S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 subunits TRM6 or TRM61
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Homo sapiens
-
HEK-293T cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
MCF-10A cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
neuroblastoma cell from adrenal gland Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in tRNA modification at the conserved nucleotide 58 in the TCC loop Homo sapiens S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?

Subunits

Subunits Comment Organism
More two subunit types, TRM6 or TRM61 Homo sapiens

Synonyms

Synonyms Comment Organism
m1A58-methyltransferase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction siRNA knockdown of either subunit of the m1A58-methyltransferase results in a slow-growth phenotype, and a marked increase in the amount of m1A58 hypomodified tRNAs. Most m1A58 hypomodified tRNAs can associate with polysomes in varying extents Homo sapiens
additional information microarray method genomic approach to determine the presence of m1A58 hypomodified tRNAs, on the basis of their permissiveness in primer extension, in human cell lines, overview. A58 hypomodification affects stability and involvement of tRNAs in translation. No hypomodification of initiator-tRNAMet. The pattern of the m1A58 hypomodified tRNAs is similar in five human cell lines Homo sapiens
physiological function N1-adenine58 methylation of initiator-tRNAMet is required for stabilizing this tRNA in human cells Homo sapiens