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Literature summary for 2.1.1.220 extracted from

  • Qiu, X.; Huang, K.; Ma, J.; Gao, Y.
    Crystallization and preliminary X-ray diffraction crystallographic study of tRNA m1A58 methyltransferase from Saccharomyces cerevisiae (2011), Acta Crystallogr. Sect. F, 67, 1448-1450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.220

Cloned(Commentary)

Cloned (Comment) Organism
genes GCD10 and GCD14 encoding TRM6 and TRM61, expression of His-tagged proteins in Escherichia coli strain Rosetta (DE3) Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged TRM6-TRM61 complex, from 0.1 M KCl, 0.1 M Tris-HCl, pH 8.0, 25% w/v PEG 2000 MME, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus TRM6-TRM61 complex Saccharomyces cerevisiae 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in tRNA Saccharomyces cerevisiae TRM6 and TRM61 which compose a tRNA m1A58 methyltransferase that methylates m1A58 in tRNAs, especially tRNAiMe S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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 genes GCD10 and GCD14 encode TRM6 and TRM61, respectively
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged TRM6 and TRM61 from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + adenine58 in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA TRM61 is the subunit that binds S-adenosyl-l-methionine and both subunits contribute to target tRNA binding, catalytic mechanism, overview Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in tRNA TRM6 and TRM61 which compose a tRNA m1A58 methyltransferase that methylates m1A58 in tRNAs, especially tRNAiMe Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?
S-adenosyl-L-methionine + adenine58 in tRNA adenine at position 58 is the most conserved nucleoside in tRNA and is located in the TpsiC loop Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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 ?

Synonyms

Synonyms Comment Organism
tRNA m1A58 methyltransferase
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 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function TRM6 and TRM61 compose a tRNA methyltransferase which catalyzes the methylation of the N1 of adenine at position 58 in tRNAs, especially initiator methionine tRNA. The TRM6-TRM61 complex is required specifically in the initiation step of translation because of a strong requirement for m1A58 in the processing and accumulation of tRNAiMet. It is also required for repression of translation of GCN4, a gene encoding a transcriptional activator of amino-acid biosynthetic enzymes, mRNA Saccharomyces cerevisiae