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Literature summary for 2.1.1.220 extracted from
- Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m1A58 tRNA methyltransferase (2004), Nucleic Acids Res., 32, 1018-1027.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1-10 mM, decrease in methylation rate. 95% inhibition at 10 mM | Mycobacterium tuberculosis |  |
| additional information | methylation at A58 is not affected by the presence of ribothymidine at position 54 | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
4 * 31000, SDS-PAGE | Mycobacterium tuberculosis |
| 115000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + adenine58 in tRNA | specific for the A58 position. Rv2118p does not methylate other positions in tRNA to any significant extent | Mycobacterium tuberculosis | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 31000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| m1A58 tRNA methyltransferase | - |
Mycobacterium tuberculosis |
| Rv2118p | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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