Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28582 | - |
4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) | Thermus thermophilus |
150000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q8GBB2 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q8GBB2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein | Thermus thermophilus | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNA | catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
TrmI | - |
Thermus thermophilus |
tRNA (m1A58) methyltransferase | - |
Thermus thermophilus |
tRNA (m1A58) MTase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of the Thermus thermophilus trmI gene results in a thermosensitive phenotype (growth defect at 80°C) | Thermus thermophilus |
physiological function | role of the N1-methylation of tRNA adenosine-58 in adaptation of life to extreme temperatures | Thermus thermophilus |