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Literature summary for 2.1.1.220 extracted from

  • Anderson, J.; Phan, L.; Hinnebusch, A.G.
    The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae (2000), Proc. Natl. Acad. Sci. USA, 97, 5173-5178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.220

Protein Variants

Protein Variants Comment Organism
additional information mutations in the predicted AdoMet-binding domain destroyed GCD14 function in vivo and (m1A)MTase activity in vitro Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000025
-
 adenine58 in initiator tRNAMet pH 8.0, 30°C, purified FlagGcd10pyGcd14p complex Saccharomyces cerevisiae
0.005
-
 S-adenosyl-L-methionine pH 8.0, 30°C, purified FlagGcd10pyGcd14p complex Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P46959 and P41814 P46959: subunit Gcd14p (catalytic subunit), P41814: subunit Gcd10p (non-catalytic subunit)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
-
 ?
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet purified Flag-tagged Gcd14p alone had no enzymatic activity and is severely impaired for tRNA-binding compared with the wild-type complex, suggesting that Gcd10p is required for tight binding of the tRNA substrate Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
-
 ?

Subunits

Subunits Comment Organism
More Gcd10p and Gcd14p function directly in m1A formation in yeast tRNAs. Purified Gcd14p alone had no enzymatic activity and is defective for tRNA binding compared with the Gcd14pyGcd10p complex. Gcd10p is required for tight binding of the tRNA substrate Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Gcd10p non-catalytic subunit Saccharomyces cerevisiae
Gcd10pyGcd14p complex
-
 Saccharomyces cerevisiae
Gcd14p catalytic subunit Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the absence of m1A from all tRNAs in Saccharomyces cerevisiae mutants lacking Gcd10p elicits severe defects in processing and stability of initiator methionine tRNA Saccharomyces cerevisiae