BRENDA - Enzyme Database show
show all sequences of 2.1.1.217

The YqfN protein of Bacillus subtilis is the tRNA: m1A22 methyltransferase (TrmK)

Roovers, M.; Kaminska, K.H.; Tkaczuk, K.L.; Gigot, D.; Droogmans, L.; Bujnicki, J.M.; Nucleic Acids Res. 36, 3252-3262 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenine22 in tRNA
Bacillus subtilis
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P54471
-
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser)
crude Escherichia coli tRNA is used, since it does not contain m1A22
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser)
-
-
-
?
S-adenosyl-L-methionine + adenine22 in tRNA
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
-
?
S-adenosyl-L-methionine + adenine22 in tRNA
crude Escherichia coli tRNA is used, since it does not contain m1A22
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + adenine22 in tRNA
Bacillus subtilis
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser)
crude Escherichia coli tRNA is used, since it does not contain m1A22
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser)
-
-
-
?
S-adenosyl-L-methionine + adenine22 in tRNA
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
-
?
S-adenosyl-L-methionine + adenine22 in tRNA
crude Escherichia coli tRNA is used, since it does not contain m1A22
713141
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Other publictions for EC 2.1.1.217
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713478
Ta
Crystal structure of Streptoco ...
Streptococcus pneumoniae
Protein Sci.
19
617-624
2010
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713141
Roovers
The YqfN protein of Bacillus s ...
Bacillus subtilis
Nucleic Acids Res.
36
3252-3262
2008
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