BRENDA - Enzyme Database show
show all sequences of 2.1.1.216

RNA polymerase III output is functionally linked to tRNA dimethyl-G26 modification

Arimbasseri, A.G.; Blewett, N.H.; Iben, J.R.; Lamichhane, T.N.; Cherkasova, V.; Hafner, M.; Maraia, R.J.; PLoS Genet. 11, e1005671 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
RNA polymerase III mutations associated with hypomyelinating leukodystrophy decrease tRNA transcription, increase of N2,N2-dimethyl G26 modification efficiency and reverse antisuppression
Schizosaccharomyces pombe
Rapamycin
a decrease in tRNA synthesis by treatment with rapamycin leads to increased N2,N2-dimethyl G26 modification
Schizosaccharomyces pombe
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4 S-adenosyl-L-methionine + guanine26 in tRNA
Schizosaccharomyces pombe
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Schizosaccharomyces pombe
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4 S-adenosyl-L-methionine + guanine26 in tRNA
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Schizosaccharomyces pombe
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
RNA polymerase III mutations associated with hypomyelinating leukodystrophy decrease tRNA transcription, increase of N2,N2-dimethyl G26 modification efficiency and reverse antisuppression
Schizosaccharomyces pombe
Rapamycin
a decrease in tRNA synthesis by treatment with rapamycin leads to increased N2,N2-dimethyl G26 modification
Schizosaccharomyces pombe
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Schizosaccharomyces pombe
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4 S-adenosyl-L-methionine + guanine26 in tRNA
Schizosaccharomyces pombe
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
Schizosaccharomyces pombe
-
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4 S-adenosyl-L-methionine + guanine26 in tRNA
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
-
737063
Schizosaccharomyces pombe
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
little changes occur in the relative levels of different tRNAs in maf1DELTA cells. By contrast, the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression
Schizosaccharomyces pombe
metabolism
global tRNA gene activation occurs with derepression of RNA polymerase III via maf1-deletion and is accompanied by a paradoxical loss of tRNA-mediated nonsense suppressor activity, manifested as an antisuppression phenotype. maf1-Antisuppression also occurs in the fission yeast amidst general activation of RNA polymerase III. Dimethyl-guanine-26 varies in the efficiency by which it is added to its target tRNAs, in a manner that is dependent on the overall activity rate of RNA polyymerase III. Overexpression of Trm1, which produces the N2,N2-dimethyl G26 modification, reverses maf1-antisuppression. Competition by increased tRNA levels in maf1DELTA cells leads to N2,N2-dimethyl G26 hypomodification due to limiting Trm1, reducing the activity of suppressor-tRNASerUCA and accounting for antisuppression
Schizosaccharomyces pombe
physiological function
the N2,N2-dimethyl G26 modification activates the tRNA for function to translate the code. The N2,N2-dimethyl G26 modification is required for the suppressor activity of sup-tRNASerUCA. The enzyme activity is regulated by nutrient/growth conditions
Schizosaccharomyces pombe
General Information (protein specific)
General Information
Commentary
Organism
malfunction
little changes occur in the relative levels of different tRNAs in maf1DELTA cells. By contrast, the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression
Schizosaccharomyces pombe
metabolism
global tRNA gene activation occurs with derepression of RNA polymerase III via maf1-deletion and is accompanied by a paradoxical loss of tRNA-mediated nonsense suppressor activity, manifested as an antisuppression phenotype. maf1-Antisuppression also occurs in the fission yeast amidst general activation of RNA polymerase III. Dimethyl-guanine-26 varies in the efficiency by which it is added to its target tRNAs, in a manner that is dependent on the overall activity rate of RNA polyymerase III. Overexpression of Trm1, which produces the N2,N2-dimethyl G26 modification, reverses maf1-antisuppression. Competition by increased tRNA levels in maf1DELTA cells leads to N2,N2-dimethyl G26 hypomodification due to limiting Trm1, reducing the activity of suppressor-tRNASerUCA and accounting for antisuppression
Schizosaccharomyces pombe
physiological function
the N2,N2-dimethyl G26 modification activates the tRNA for function to translate the code. The N2,N2-dimethyl G26 modification is required for the suppressor activity of sup-tRNASerUCA. The enzyme activity is regulated by nutrient/growth conditions
Schizosaccharomyces pombe
Other publictions for EC 2.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737063
Arimbasseri
RNA polymerase III output is f ...
Schizosaccharomyces pombe
PLoS Genet.
11
e1005671
2015
2
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1
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4
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1
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4
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1
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2
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1
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1
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4
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4
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3
3
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-
729858
Kawamura
Transfer RNA methyltransferase ...
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
-
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1
-
-
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-
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3
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8
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1
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12
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1
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1
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1
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1
1
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3
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1
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12
-
1
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1
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-
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1
1
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-
688425
Ihsanawati; Nishimoto
Crystal Structure of tRNA N(2) ...
Pyrococcus horikoshii
J. Mol. Biol.
383
871-884
2008
-
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1
-
-
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-
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1
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1
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3
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1
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1
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3
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-
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-
712100
Liu
Caenorhabditis elegans ZC376.5 ...
Caenorhabditis elegans, Saccharomyces cerevisiae
Gene
226
73-81
1999
-
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1
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3
-
-
-
-
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2
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7
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1
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6
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1
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1
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3
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2
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1
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6
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1
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-
712733
Constantinesco
Characterisation and enzymatic ...
Pyrococcus furiosus
J. Mol. Biol.
291
375-392
1999
-
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1
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-
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1
-
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2
3
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1
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1
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1
8
1
1
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3
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1
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1
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2
3
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1
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1
8
1
1
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3
-
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-
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-
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-
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-
713134
Constantinesco
The tRNA(guanine-26,N2-N2) met ...
Pyrococcus furiosus
Nucleic Acids Res.
26
3753-3761
1998
-
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1
-
-
-
-
-
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1
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9
-
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-
-
-
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3
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1
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1
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1
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1
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-
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3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713135
Liu
Point and deletion mutations e ...
Saccharomyces cerevisiae
Nucleic Acids Res.
26
5102-5108
1998
-
-
1
-
5
-
-
-
-
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1
-
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3
-
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1
-
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-
-
2
3
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1
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1
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5
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1
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1
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-
2
3
-
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-
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-
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1
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-
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-
711185
Edqvist
Structural elements in yeast t ...
Saccharomyces cerevisiae
Biochemistry
33
9546-9551
1994
-
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1
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3
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-
-
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-
713133
Edqvist
Identity elements for N2-dimet ...
Saccharomyces cerevisiae
Nucleic Acids Res.
20
6575-6581
1992
-
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1
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1
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1
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