BRENDA - Enzyme Database show
show all sequences of 2.1.1.216

Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase

Liu, J.; Liu, J.; Straby, K.B.; Nucleic Acids Res. 26, 5102-5108 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli and the His-tagged Trm1 protein
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
E47A
mutant enzyme shows 6% of wild-type activity
Saccharomyces cerevisiae
G48A
mutant enzyme shows 16% of wild-type activity
Saccharomyces cerevisiae
additional information
comparatively short N- or C-terminal deletions from the 570 amino acid long Trm1 polypeptide decreased or eliminated the enzyme activity. The first 27 N-terminal amino acids in rTrmp1 are not needed for G26 modification activity in vitro or in vivo. DELTAC5aa causes the in vitro modification activity to drastically decrease to 27% of the wild-type rate. Additional deletions up to DELTA30aa further lower the activity to about 7% of the wild-type value. In DELTA10aa an increase of the N2-methylguanine26 relative to N2-dimethylguanine26 tRNA is observed, but with further C-terminal deletions the monomethylation activity is also severely lowered
Saccharomyces cerevisiae
S467L
complete loss of enzyme activity in vitro against trm1 yeast tRNA
Saccharomyces cerevisiae
S570A
fully active mutant enzyme
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64050
-
calculated from sequence
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
P15565
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
4°C, the enzyme precipitates if stored for several days
Saccharomyces cerevisiae
frozen in small portions, enzyme maintains activity for at least several months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNA
wild-type enzyme produces 94% N2-dimethylguanine26 tRNA and 6% N2-methylguanine26 in tRNA
713135
Saccharomyces cerevisiae
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
713135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
713135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
calculated from sequence
-
9.2
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli and the His-tagged Trm1 protein
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E47A
mutant enzyme shows 6% of wild-type activity
Saccharomyces cerevisiae
G48A
mutant enzyme shows 16% of wild-type activity
Saccharomyces cerevisiae
additional information
comparatively short N- or C-terminal deletions from the 570 amino acid long Trm1 polypeptide decreased or eliminated the enzyme activity. The first 27 N-terminal amino acids in rTrmp1 are not needed for G26 modification activity in vitro or in vivo. DELTAC5aa causes the in vitro modification activity to drastically decrease to 27% of the wild-type rate. Additional deletions up to DELTA30aa further lower the activity to about 7% of the wild-type value. In DELTA10aa an increase of the N2-methylguanine26 relative to N2-dimethylguanine26 tRNA is observed, but with further C-terminal deletions the monomethylation activity is also severely lowered
Saccharomyces cerevisiae
S467L
complete loss of enzyme activity in vitro against trm1 yeast tRNA
Saccharomyces cerevisiae
S570A
fully active mutant enzyme
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64050
-
calculated from sequence
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
4°C, the enzyme precipitates if stored for several days
Saccharomyces cerevisiae
frozen in small portions, enzyme maintains activity for at least several months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNA
wild-type enzyme produces 94% N2-dimethylguanine26 tRNA and 6% N2-methylguanine26 in tRNA
713135
Saccharomyces cerevisiae
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
713135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
713135
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
calculated from sequence
-
9.2
Other publictions for EC 2.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737063
Arimbasseri
RNA polymerase III output is f ...
Schizosaccharomyces pombe
PLoS Genet.
11
e1005671
2015
2
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1
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4
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1
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4
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1
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2
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1
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1
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4
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4
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-
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-
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3
3
-
-
-
729858
Kawamura
Transfer RNA methyltransferase ...
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
-
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1
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3
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8
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1
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12
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1
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1
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1
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1
1
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3
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1
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12
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1
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1
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1
1
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688425
Ihsanawati; Nishimoto
Crystal Structure of tRNA N(2) ...
Pyrococcus horikoshii
J. Mol. Biol.
383
871-884
2008
-
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1
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1
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1
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1
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3
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712100
Liu
Caenorhabditis elegans ZC376.5 ...
Caenorhabditis elegans, Saccharomyces cerevisiae
Gene
226
73-81
1999
-
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1
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3
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-
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2
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7
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1
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6
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1
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1
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2
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1
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6
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1
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712733
Constantinesco
Characterisation and enzymatic ...
Pyrococcus furiosus
J. Mol. Biol.
291
375-392
1999
-
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1
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1
-
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2
3
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1
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1
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1
8
1
1
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3
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1
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1
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2
3
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1
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1
8
1
1
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3
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-
713134
Constantinesco
The tRNA(guanine-26,N2-N2) met ...
Pyrococcus furiosus
Nucleic Acids Res.
26
3753-3761
1998
-
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1
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1
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9
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3
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1
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1
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1
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1
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3
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1
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1
-
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-
-
-
-
-
-
-
713135
Liu
Point and deletion mutations e ...
Saccharomyces cerevisiae
Nucleic Acids Res.
26
5102-5108
1998
-
-
1
-
5
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
2
3
-
-
-
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-
-
-
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-
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1
-
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1
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-
5
-
-
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-
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1
-
-
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1
-
-
-
2
3
-
-
-
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-
-
-
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1
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-
711185
Edqvist
Structural elements in yeast t ...
Saccharomyces cerevisiae
Biochemistry
33
9546-9551
1994
-
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1
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3
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-
-
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-
713133
Edqvist
Identity elements for N2-dimet ...
Saccharomyces cerevisiae
Nucleic Acids Res.
20
6575-6581
1992
-
-
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1
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1
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1
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