BRENDA - Enzyme Database show
show all sequences of 2.1.1.216

The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli

Constantinesco, F.; Benachenhou, N.; Motorin, Y.; Grosjean, H.; Nucleic Acids Res. 26, 3753-3761 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43300
-
calculated from sequence
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
P15565
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43300
-
calculated from sequence
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe
tRNAPhe from yeast
713134
Pyrococcus furiosus
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Pyrococcus furiosus
Other publictions for EC 2.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737063
Arimbasseri
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PLoS Genet.
11
e1005671
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2
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1
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4
-
1
-
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-
-
-
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-
4
-
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-
-
-
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1
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2
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1
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1
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4
-
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-
-
-
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-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
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-
729858
Kawamura
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Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
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1
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-
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-
-
-
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3
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8
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1
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-
-
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12
-
1
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1
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1
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1
1
-
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3
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1
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
688425
Ihsanawati; Nishimoto
Crystal Structure of tRNA N(2) ...
Pyrococcus horikoshii
J. Mol. Biol.
383
871-884
2008
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
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3
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1
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1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712100
Liu
Caenorhabditis elegans ZC376.5 ...
Caenorhabditis elegans, Saccharomyces cerevisiae
Gene
226
73-81
1999
-
-
1
-
3
-
-
-
-
-
2
-
-
7
-
-
1
-
-
-
-
-
6
-
-
-
-
-
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-
-
-
-
1
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1
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3
-
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2
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-
-
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1
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
712733
Constantinesco
Characterisation and enzymatic ...
Pyrococcus furiosus
J. Mol. Biol.
291
375-392
1999
-
-
1
-
-
-
-
1
-
-
2
3
-
1
-
-
1
-
-
-
-
1
8
1
1
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
3
-
-
-
1
-
-
-
1
8
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
713134
Constantinesco
The tRNA(guanine-26,N2-N2) met ...
Pyrococcus furiosus
Nucleic Acids Res.
26
3753-3761
1998
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
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1
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-
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-
-
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1
-
-
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-
-
-
-
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3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713135
Liu
Point and deletion mutations e ...
Saccharomyces cerevisiae
Nucleic Acids Res.
26
5102-5108
1998
-
-
1
-
5
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
711185
Edqvist
Structural elements in yeast t ...
Saccharomyces cerevisiae
Biochemistry
33
9546-9551
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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3
-
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-
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-
-
-
-
-
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-
-
-
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713133
Edqvist
Identity elements for N2-dimet ...
Saccharomyces cerevisiae
Nucleic Acids Res.
20
6575-6581
1992
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
-
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1
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1
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