BRENDA - Enzyme Database show
show all sequences of 2.1.1.216

Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs

Edqvist, J.; Grosjean, H.; Straby, K.B.; Nucleic Acids Res. 20, 6575-6581 (1992)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in yeast tRNAAsp G26 is unmodified. Successive change of the near surroundings of G26 in this tRNA is performed until G26 becomes modified to N2-dimethylguanine26 tRNA by a tRNA(m2(2)G26)methyltransferase in Xenopus laevis oocytes. In this the two D-stem basepairs C1 -G24, G10-C25 are identified immediately preceding G26 as major identity elements for the dimethylating enzyme modifying G26. Increasing the extra loop in tRNAAsp from four to the more usual five bases influence the global structure of the tRNA such that the mJG26 formation is drastically decreased even if the near region of G26 had the two consensus basepairs. Not only are the two consensus base pairs in the D-stem a prerequisite for G26 modification, but also is any part of the tRNA molecule that influence the 3D-structure important for the recognition between nuclear coded tRNAs and the tRNA(mJG26)methyltransferase
713133
Saccharomyces cerevisiae
?
-
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
in yeast tRNAAsp G26 is unmodified. Successive change of the near surroundings of G26 in this tRNA is performed until G26 becomes modified to N2-dimethylguanine26 tRNA by a tRNA(m2(2)G26)methyltransferase in Xenopus laevis oocytes. In this the two D-stem basepairs C1 -G24, G10-C25 are identified immediately preceding G26 as major identity elements for the dimethylating enzyme modifying G26. Increasing the extra loop in tRNAAsp from four to the more usual five bases influence the global structure of the tRNA such that the mJG26 formation is drastically decreased even if the near region of G26 had the two consensus basepairs. Not only are the two consensus base pairs in the D-stem a prerequisite for G26 modification, but also is any part of the tRNA molecule that influence the 3D-structure important for the recognition between nuclear coded tRNAs and the tRNA(mJG26)methyltransferase
713133
Saccharomyces cerevisiae
?
-
-
-
-
Other publictions for EC 2.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737063
Arimbasseri
RNA polymerase III output is f ...
Schizosaccharomyces pombe
PLoS Genet.
11
e1005671
2015
2
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
729858
Kawamura
Transfer RNA methyltransferase ...
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
-
-
1
-
-
-
-
-
-
-
-
3
-
8
-
-
1
-
-
-
-
-
12
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
688425
Ihsanawati; Nishimoto
Crystal Structure of tRNA N(2) ...
Pyrococcus horikoshii
J. Mol. Biol.
383
871-884
2008
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712100
Liu
Caenorhabditis elegans ZC376.5 ...
Caenorhabditis elegans, Saccharomyces cerevisiae
Gene
226
73-81
1999
-
-
1
-
3
-
-
-
-
-
2
-
-
7
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
712733
Constantinesco
Characterisation and enzymatic ...
Pyrococcus furiosus
J. Mol. Biol.
291
375-392
1999
-
-
1
-
-
-
-
1
-
-
2
3
-
1
-
-
1
-
-
-
-
1
8
1
1
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
3
-
-
-
1
-
-
-
1
8
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
713134
Constantinesco
The tRNA(guanine-26,N2-N2) met ...
Pyrococcus furiosus
Nucleic Acids Res.
26
3753-3761
1998
-
-
1
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713135
Liu
Point and deletion mutations e ...
Saccharomyces cerevisiae
Nucleic Acids Res.
26
5102-5108
1998
-
-
1
-
5
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
711185
Edqvist
Structural elements in yeast t ...
Saccharomyces cerevisiae
Biochemistry
33
9546-9551
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713133
Edqvist
Identity elements for N2-dimet ...
Saccharomyces cerevisiae
Nucleic Acids Res.
20
6575-6581
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-