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Literature summary for 2.1.1.216 extracted from
- Characterisation and enzymatic properties of tRNA(guanine 26, N2,N2)-dimethyltransferase (Trm1p) from Pyrococcus furiosus (1999), J. Mol. Biol., 291, 375-392.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
S-adenosyl-L-methionine | pH 7.2, 50°C | Pyrococcus furiosus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45700 | - |
calculated from sequence | Pyrococcus furiosus |
| 49000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + guanine26 in tRNA | Pyrococcus furiosus | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine26 in tRNA | Pyrococcus furiosus | - |
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA | - |
? | |
| S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | Pyrococcus furiosus | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme (pfTrm1p) with a His6-tag at the N-terminus | Pyrococcus furiosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, the purified enzyme can be stored for several months without loss of activity | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + guanine26 in tRNA | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | Pyrococcus furiosus | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
| 2 S-adenosyl-L-methionine + guanine26 in tRNA | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions | Pyrococcus furiosus | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
| 2 S-adenosyl-L-methionine + guanine26 in tRNAPhe | tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | Pyrococcus furiosus | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe | - |
? | |
| S-adenosyl-L-methionine + guanine26 in tRNA | - |
Pyrococcus furiosus | S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine26 in tRNAPhe | tRNAPhe from yeast | Pyrococcus furiosus | S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe | - |
? | |
| S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | Pyrococcus furiosus | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? | |
| S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions | Pyrococcus furiosus | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? | |
| S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe | tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group | Pyrococcus furiosus | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trm1p | - |
Pyrococcus furiosus |
| tRNA(guanine26,N2,N2)-dimethyltransferase | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
2 h: stable | Pyrococcus furiosus |
| 90 | - |
2 h: 30% loss of activity | Pyrococcus furiosus |
| 95 | - |
t1/2: 2 h | Pyrococcus furiosus |
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