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Literature summary for 2.1.1.216 extracted from

  • Liu, J.; Zhou, G.Q.; Strabym K.B.
    Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity (1999), Gene, 226, 73-81.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
K290A mutant enzyme loses 56% of its methylation activity Saccharomyces cerevisiae
R246A more than 97% loss of activity Caenorhabditis elegans
R246G mutation eliminates the methylation activity Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58400
-
calculated from sequence Caenorhabditis elegans
59000
-
SDS-PAGE Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q23270
-
-
Saccharomyces cerevisiae P15565
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
Saccharomyces cerevisiae 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA Caenorhabditis elegans 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
Caenorhabditis elegans S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
Caenorhabditis elegans S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
?

Synonyms

Synonyms Comment Organism
Trm1p
-
Saccharomyces cerevisiae
tRNA(m22G26)dimethyltransferase
-
Saccharomyces cerevisiae
tRNA(m22G26)dimethyltransferase
-
Caenorhabditis elegans
ZC376.5
-
Caenorhabditis elegans

pI Value

Organism Comment pI Value Maximum pI Value
Caenorhabditis elegans calculated from sequence
-
8.7