BRENDA - Enzyme Database show
show all sequences of 2.1.1.216

Crystal Structure of tRNA N(2),N(2)-Guanosine Dimethyltransferase Trm1 from Pyrococcus horikoshii

Ihsanawati; Nishimoto, M.; Higashijima, K.; Shirouzu, M.; Grosjean, H.; Bessho, Y.; Yokoyama, S.; J. Mol. Biol. 383, 871-884 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging drop method, crystal structures of Trm1 from Pyrococcus horikoshii liganded with S-adenosyl-l-methionine or S-adenosyl-l-homocysteine
Pyrococcus horikoshii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus horikoshii
O59493
-
-
Purification (Commentary)
Commentary
Organism
native and mutant Trm1 proteins
Pyrococcus horikoshii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
688425
Pyrococcus horikoshii
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
688425
Pyrococcus horikoshii
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
688425
Pyrococcus horikoshii
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop method, crystal structures of Trm1 from Pyrococcus horikoshii liganded with S-adenosyl-l-methionine or S-adenosyl-l-homocysteine
Pyrococcus horikoshii
Purification (Commentary) (protein specific)
Commentary
Organism
native and mutant Trm1 proteins
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
688425
Pyrococcus horikoshii
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
-
688425
Pyrococcus horikoshii
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
688425
Pyrococcus horikoshii
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
Other publictions for EC 2.1.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Ihsanawati; Nishimoto
Crystal Structure of tRNA N(2) ...
Pyrococcus horikoshii
J. Mol. Biol.
383
871-884
2008
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1
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1
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712733
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291
375-392
1999
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3
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1
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1
8
1
1
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1
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3
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1
8
1
1
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3
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713134
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The tRNA(guanine-26,N2-N2) met ...
Pyrococcus furiosus
Nucleic Acids Res.
26
3753-3761
1998
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1
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1
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9
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1
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1
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1
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3
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1
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1
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713135
Liu
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Nucleic Acids Res.
26
5102-5108
1998
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1
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5
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1
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3
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1
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1
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5
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Edqvist
Structural elements in yeast t ...
Saccharomyces cerevisiae
Biochemistry
33
9546-9551
1994
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Edqvist
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Saccharomyces cerevisiae
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20
6575-6581
1992
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1
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1
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1
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