Literature summary for 2.1.1.214 extracted from

Purushothaman, S.K.; Bujnicki, J.M.; Grosjean, H.; Lapeyre, B.
Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA (2005), Mol. Cell. Biol., 25, 4359-4370.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant Trm11p expressed in Escherichia coli exhibited no m2G10 formation activity. Trm112p and Trm11p are both required for the formation of m2G10 in vivo as well as in vitro	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Saccharomyces cerevisiae	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	the enzyme is composed of at least two subunits that are associated in vivo: Trm11p, which is the catalytic subunit, and Trm112p, a putative zinc-binding protein	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12463	catalytic subunit	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine10 in tRNA	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA	-	?

Subunits

Subunits	Comment	Organism
More	composed of at least two subunits that are associated in vivo: Trm11p (Yol124c), which is the catalytic subunit, and Trm112p (Ynr046w), a putative zinc-binding protein. While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell. Trm112p is associated with at least four proteins: two tRNA methyltransferases (Trm9p and Trm11p), one putative protein methyltransferase (Mtc6p/Ydr140w), and one protein with a Rossmann fold dehydrogenase domain (Lys9p/Ynr050c)	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Trm11p	catalytic subunit	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)