BRENDA - Enzyme Database
show all sequences of 2.1.1.214

Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA

Purushothaman, S.K.; Bujnicki, J.M.; Grosjean, H.; Lapeyre, B.; Mol. Cell. Biol. 25, 4359-4370 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant Trm11p expressed in Escherichia coli exhibited no m2G10 formation activity. Trm112p and Trm11p are both required for the formation of m2G10 in vivo as well as in vitro
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Saccharomyces cerevisiae
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zinc
the enzyme is composed of at least two subunits that are associated in vivo: Trm11p, which is the catalytic subunit, and Trm112p, a putative zinc-binding protein
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
Q12463
catalytic subunit
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + guanine10 in tRNA
-
675961
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
composed of at least two subunits that are associated in vivo: Trm11p (Yol124c), which is the catalytic subunit, and Trm112p (Ynr046w), a putative zinc-binding protein. While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell. Trm112p is associated with at least four proteins: two tRNA methyltransferases (Trm9p and Trm11p), one putative protein methyltransferase (Mtc6p/Ydr140w), and one protein with a Rossmann fold dehydrogenase domain (Lys9p/Ynr050c)
Saccharomyces cerevisiae
Synonyms
Synonyms
Commentary
Organism
Trm11p
catalytic subunit
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant Trm11p expressed in Escherichia coli exhibited no m2G10 formation activity. Trm112p and Trm11p are both required for the formation of m2G10 in vivo as well as in vitro
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Saccharomyces cerevisiae
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zinc
the enzyme is composed of at least two subunits that are associated in vivo: Trm11p, which is the catalytic subunit, and Trm112p, a putative zinc-binding protein
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + guanine10 in tRNA
-
675961
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
composed of at least two subunits that are associated in vivo: Trm11p (Yol124c), which is the catalytic subunit, and Trm112p (Ynr046w), a putative zinc-binding protein. While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell. Trm112p is associated with at least four proteins: two tRNA methyltransferases (Trm9p and Trm11p), one putative protein methyltransferase (Mtc6p/Ydr140w), and one protein with a Rossmann fold dehydrogenase domain (Lys9p/Ynr050c)
Saccharomyces cerevisiae
General Information
General Information
Commentary
Organism
malfunction
While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell
Saccharomyces cerevisiae
Other publictions for EC 2.1.1.214
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712436
Mazauric
Trm112p is a 15-kDa zinc finge ...
Saccharomyces cerevisiae
J. Biol. Chem.
285
18505-18515
2010
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705993
Okada
Production of yeast (m2G10) me ...
Saccharomyces cerevisiae
Nucleic Acids Symp. Ser.
53
303-304
2009
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675961
Purushothaman
Trm11p and Trm112p are both re ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
25
4359-4370
2005
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