Literature summary for 2.1.1.212 extracted from

Deavours, B.E.; Liu, C.J.; Naoumkina, M.A.; Tang, Y.; Farag, M.A.; Sumner, L.W.; Noel, J.P.; Dixon, R.A.
Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula (2006), Plant Mol. Biol., 62, 715-733.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Medicago truncatula

Organism

Organism	UniProt	Comment	Textmining
Medicago truncatula	Q29U70	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Medicago truncatula

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	low activity with dihydrodaidzein. 4'-Methoxy, 7-methoxy, and 4',7-dimethoxy products are formed in the reaction with dihydrodaidzein. No activity with: 6,7,4'-trihydroxyisoflavone, 6,7'-dihydroxy,4'-methoxyisoflavone, 7,3',4'-trihydroxyisoflavone, daidzein, genistein, glycitein, coumestrol, apigenin, 7,4'-dihydroxyflavone and myricetin	Medicago truncatula	?	-	?
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone	-	Medicago truncatula	S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone	-	?

Synonyms

Synonyms	Comment	Organism
MtIOMT5	-	Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Medicago truncatula

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Medicago truncatula

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Release 2023.1 (January 2023)