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Literature summary for 2.1.1.210 extracted from

  • Badenhop, F.; Steiger, S.; Sandmann, M.; Sandmann, G.
    Expression and biochemical characterization of the 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus (2003), FEMS Microbiol. Lett., 222, 237-242.
    View publication on PubMed
Search for more literature for 2.1.1.210

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli in an active form Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0266
-
 1'-hydroxydemethylspheroidene pH 8.0, 28°C Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene Rhodobacter capsulatus the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene Rhodobacter capsulatus the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene
-
 ?
S-adenosyl-L-methionine + 1-hydroxyneurosporene Rhodobacter capsulatus the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus S-adenosyl-L-homocysteine + 1-methoxyneurosporene
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme converts not only all possible 1-hydroxycarotenoids in the spheroidene/1'-hydroxyspheroidene biosynthetic pathway of Rhodobacter capsulatus but also carotenoid intermediates leading to the formation of spirilloxanthin in a pathway which is absent in Rhodobacter capsulatus but present in related species. Hydroxycarotenoids not accepted by CrtF are 1'-hydroxy-3,4-didehydrolycopene, 1-hydroxy-zeta-carotene, 1,1'-dihydroxy-zeta-carotene both without the 7,8- and 7',8'-double bond and cyclic zeaxanthin. From all these complementation results the structural requirements of a substrate for CrtF can be established. The 1-hydroxy-carotenoid should be acyclic with a 5,6-double bond in conjugation with the central polyene chain. A 3,4-double bond is optional. The other end group can be quite variable without a negative impact on methylation, provided that it is not a fully desaturated psi-end group Rhodobacter capsulatus ?
-
 ?
S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene
-
 Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene
-
 ?
S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopene not involved in the carotenogenic pathway of Rhodobacter capsulatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1,1'-dimethoxy-3,4,3',4'-tetradehydrolycopene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxy-3,4-didehydrolycopene not involved in the carotenogenic pathway of Rhodobacter capsulatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1,1'-dimethoxy-3,4-didehydrolycopene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxylycopene not involved in the carotenogenic pathway of Rhodobacter capsulatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1,1'-dimethoxylycopene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene
-
 Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene
-
 ?
S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene
-
 ?
S-adenosyl-L-methionine + 1-hydroxy-3,4-didehydrolycopene not involved in the carotenogenic pathway of Rhodobacter capsulatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxy-3,4-didehydrolycopene
-
 ?
S-adenosyl-L-methionine + 1-hydroxylycopene not involved in the carotenogenic pathway of Rhodobacter capsulatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxylycopene
-
 ?
S-adenosyl-L-methionine + 1-hydroxyneurosporene
-
 Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxyneurosporene
-
 ?
S-adenosyl-L-methionine + 1-hydroxyneurosporene the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus Rhodobacter capsulatus S-adenosyl-L-homocysteine + 1-methoxyneurosporene
-
 ?
S-adenosyl-L-methionine + demethylspheroidene
-
 Rhodobacter capsulatus S-adenosyl-L-homocysteine + spheroidene
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
1-HO-carotenoid methylase
-
 Rhodobacter capsulatus
1-hydroxycarotenoid methylase
-
 Rhodobacter capsulatus
1-hydroxycarotenoid O-methylase
-
 Rhodobacter capsulatus
CrtF
-
 Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Rhodobacter capsulatus