BRENDA - Enzyme Database show
show all sequences of 2.1.1.21

The enzymatic synthesis of N-methylglutamic acid

Shaw, W.V.; Tsai, L.; Stadtman, E.R.; J. Biol. Chem. 241, 935-945 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ammonia
competitive with respect to methylamine
Pseudomonas sp.
arsenite
-
Pseudomonas sp.
hydroxylamine
reversible
Pseudomonas sp.
iodoacetate
-
Pseudomonas sp.
N-ethylmaleimide
-
Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylamine + L-glutamate
Pseudomonas sp.
involved in one carbon metabolism
NH3 + N-methyl-L-glutamate
-
Pseudomonas sp.
r
methylamine + L-glutamate
Pseudomonas sp. AM1(2006)
involved in one carbon metabolism
NH3 + N-methyl-L-glutamate
-
Pseudomonas sp. AM1(2006)
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
no activity when grown without methylamine in growth medium
-
Pseudomonas sp. AM1(2006)
-
no activity when grown without methylamine in growth medium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylamine + L-glutamate
-
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp.
r
methylamine + L-glutamate
involved in one carbon metabolism
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp.
r
methylamine + L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
r
methylamine + L-glutamate
involved in one carbon metabolism
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
r
methylamine + L-glutamate-gamma-methylester
-
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate-gamma-methyl ester
-
-
-
r
methylamine + L-glutamate-gamma-methylester
-
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate-gamma-methyl ester
-
-
-
r
methylamine + L-glutamine
-
485233
Pseudomonas sp.
?
-
-
-
r
methylamine + L-glutamine
-
485233
Pseudomonas sp. AM1(2006)
?
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Pseudomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
49
-
ammonia
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ammonia
competitive with respect to methylamine
Pseudomonas sp.
arsenite
-
Pseudomonas sp.
hydroxylamine
reversible
Pseudomonas sp.
iodoacetate
-
Pseudomonas sp.
N-ethylmaleimide
-
Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
49
-
ammonia
-
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylamine + L-glutamate
Pseudomonas sp.
involved in one carbon metabolism
NH3 + N-methyl-L-glutamate
-
Pseudomonas sp.
r
methylamine + L-glutamate
Pseudomonas sp. AM1(2006)
involved in one carbon metabolism
NH3 + N-methyl-L-glutamate
-
Pseudomonas sp. AM1(2006)
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylamine + L-glutamate
-
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp.
r
methylamine + L-glutamate
involved in one carbon metabolism
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp.
r
methylamine + L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
r
methylamine + L-glutamate
involved in one carbon metabolism
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate
-
485233
Pseudomonas sp. AM1(2006)
r
methylamine + L-glutamate-gamma-methylester
-
485233
Pseudomonas sp.
NH3 + N-methyl-L-glutamate-gamma-methyl ester
-
-
-
r
methylamine + L-glutamate-gamma-methylester
-
485233
Pseudomonas sp. AM1(2006)
NH3 + N-methyl-L-glutamate-gamma-methyl ester
-
-
-
r
methylamine + L-glutamine
-
485233
Pseudomonas sp.
?
-
-
-
r
methylamine + L-glutamine
-
485233
Pseudomonas sp. AM1(2006)
?
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Pseudomonas sp.
Other publictions for EC 2.1.1.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700286
Latypova
Genetics of the glutamate-medi ...
Methyloversatilis universalis, Methyloversatilis universalis FAM5
Mol. Microbiol.
75
426-439
2010
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2
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5
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1
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6
-
2
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-
-
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2
-
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1
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6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
485237
Bornemann
Flavoenzymes that catalyse rea ...
Pseudomonas sp.
Nat. Prod. Rep.
19
761-772
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
-
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
2
-
-
-
-
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-
-
-
-
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-
-
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-
485236
Hersh
N-Methyl-L-glutamate synthase ...
Pseudomonas sp.
Methods Enzymol.
113
36-42
1985
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-
-
-
-
-
-
-
-
-
2
1
-
1
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1
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1
-
12
1
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-
-
1
-
-
1
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-
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-
1
-
-
-
-
-
-
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2
1
-
-
-
1
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-
1
-
12
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
485235
Schuman Jorns
N-Methylglutamate synthetase. ...
Pseudomonas sp., Pseudomonas sp. AM1(2006)
J. Biol. Chem.
250
3620-3628
1975
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-
-
-
-
-
-
-
-
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-
2
-
5
-
-
1
-
-
-
3
-
4
-
-
-
-
2
-
-
-
2
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2
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-
2
-
-
-
1
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-
3
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
485234
Pollock
N-Methylglutamate synthetase. ...
Pseudomonas sp.
J. Biol. Chem.
248
6724-6733
1973
-
-
-
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-
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2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
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-
1
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2
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1
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-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
485233
Shaw
The enzymatic synthesis of N-m ...
Pseudomonas sp., Pseudomonas sp. AM1(2006)
J. Biol. Chem.
241
935-945
1966
-
-
-
-
-
-
6
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
8
-
-
-
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-
1
1
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1
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6
1
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-
2
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8
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1
1
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