Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17726 | - |
2 * 17726, calulated from sequence | Escherichia coli |
36000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu | Escherichia coli | the enzyme methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNALeu(CmAA) and tRNALeu(cmnm5UmAA) | S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu | - |
? | |
S-adenosyl-L-methionine + cytidine34 in tRNA | Escherichia coli | the enzyme methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNALeu(CmAA) and tRNALeu(cmnm5UmAA) | S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AGJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-YibK protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu | the enzyme methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNALeu(CmAA) and tRNALeu(cmnm5UmAA) | Escherichia coli | S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu | - |
? | |
S-adenosyl-L-methionine + cytidine34 in tRNA | the enzyme methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNALeu(CmAA) and tRNALeu(cmnm5UmAA) | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA | - |
? | |
S-adenosyl-L-methionine + cytidine34 in tRNA | the enzyme methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNALeu(CmAA) and tRNALeu(cmnm5UmAA). No methylation of fully synthetic in vitro transcrips of tRNA(CAA), lacking all natural modifications present in in vivo transcripts. The motifs in tRNALeu required for YibK recognition and catalysis include the N6-(isopentenyl)-2-methylthioadenosine (ms2i6A) at position 37 and a pyridine at position 34. It has a clear preference for adenosine at position 35 | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 17726, calulated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
methyltransferase yibK | - |
Escherichia coli |
Trml | - |
Escherichia coli |
tRNA (cytidine34/5-carboxymethylaminomethyluridine34'-O)-methyltransferase | - |
Escherichia coli |
tRNA methyltransferase L | - |
Escherichia coli |
YibK | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of yibK leads to loss of 2'-O-methylation at position 34 in both tRNALeu(CmAA) and tRNALeu(cmnm5UmAA). Loss of YibK methylation reduces the efficiency of codonwobble base interaction. Inactivation of yibK has no detectable effect on steady-state growth rate, although a distinct disadvantage is noted in multiple-round, mixed-population growth experiments, suggesting that the ability to recover from the stationary phase is impaired. Methylation is restored in vivo by complementing with a recombinant copy of yibK | Escherichia coli |