BRENDA - Enzyme Database show
show all sequences of 2.1.1.204

Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine

Mueller, M.; Hartmann, M.; Schuster, I.; Bender, S.; Thuering, K.L.; Helm, M.; Katze, J.R.; Nellen, W.; Lyko, F.; Ehrenhofer-Murray, A.E.; Nucleic Acids Res. 43, 10952-10962 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
queuine
a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium
Dictyostelium discoideum
queuine
a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium
Schizosaccharomyces pombe
Cloned(Commentary)
Commentary
Organism
overexpression of GFP-tagged DnmA in Dictyostelium discoideum
Dictyostelium discoideum
Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of a DnmA-deletion mutant
Dictyostelium discoideum
additional information
generation of a Prmt1-deletion mutant
Schizosaccharomyces pombe
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Dictyostelium discoideum
Mg2+
required
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Dictyostelium discoideum
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs
?
-
-
-
additional information
Schizosaccharomyces pombe
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNA
Dictyostelium discoideum
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
Schizosaccharomyces pombe
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dictyostelium discoideum
-
-
-
Schizosaccharomyces pombe
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs
736905
Dictyostelium discoideum
?
-
-
-
-
additional information
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe
736905
Schizosaccharomyces pombe
?
-
-
-
-
additional information
in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine
736905
Schizosaccharomyces pombe
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNA
-
736905
Dictyostelium discoideum
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
736905
Dictyostelium discoideum
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
736905
Schizosaccharomyces pombe
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone
736905
Schizosaccharomyces pombe
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Dictyostelium discoideum
22
-
assay at
Schizosaccharomyces pombe
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Dictyostelium discoideum
7.5
-
assay at
Schizosaccharomyces pombe
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Dictyostelium discoideum
S-adenosyl-L-methionine
-
Schizosaccharomyces pombe
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
queuine
a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium
Dictyostelium discoideum
queuine
a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium
Schizosaccharomyces pombe
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of GFP-tagged DnmA in Dictyostelium discoideum
Dictyostelium discoideum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Dictyostelium discoideum
S-adenosyl-L-methionine
-
Schizosaccharomyces pombe
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of a DnmA-deletion mutant
Dictyostelium discoideum
additional information
generation of a Prmt1-deletion mutant
Schizosaccharomyces pombe
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Dictyostelium discoideum
Mg2+
required
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Dictyostelium discoideum
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs
?
-
-
-
additional information
Schizosaccharomyces pombe
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNA
Dictyostelium discoideum
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
Schizosaccharomyces pombe
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs
736905
Dictyostelium discoideum
?
-
-
-
-
additional information
Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe
736905
Schizosaccharomyces pombe
?
-
-
-
-
additional information
in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine
736905
Schizosaccharomyces pombe
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNA
-
736905
Dictyostelium discoideum
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
736905
Dictyostelium discoideum
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
736905
Schizosaccharomyces pombe
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone
736905
Schizosaccharomyces pombe
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Dictyostelium discoideum
22
-
assay at
Schizosaccharomyces pombe
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Dictyostelium discoideum
7.5
-
assay at
Schizosaccharomyces pombe
Expression
Organism
Commentary
Expression
Schizosaccharomyces pombe
cultivation of Schizosaccharomyces pombe in minimal medium leads to approximately 23% of tRNAAsp methylation
down
Schizosaccharomyces pombe
methylation is not enhanced by stress conditions such as glucose starvation, heat stress or oxidative stress
additional information
Schizosaccharomyces pombe
tRNAAsp methylation is induced to 100% when cells are grown in the presence of peptone
up
General Information
General Information
Commentary
Organism
malfunction
methylation at C11 of tRNASer (GCT) shows an inverse correlation to the presence of Pmt1 and queuine in pmt1-mutant cells in that its level increases from 23 to 37% in the absence of Pmt1
Schizosaccharomyces pombe
physiological function
Pmt1 provides in vivo tRNA methylation activity that is strongly controlled by nutritional cues
Schizosaccharomyces pombe
General Information (protein specific)
General Information
Commentary
Organism
malfunction
methylation at C11 of tRNASer (GCT) shows an inverse correlation to the presence of Pmt1 and queuine in pmt1-mutant cells in that its level increases from 23 to 37% in the absence of Pmt1
Schizosaccharomyces pombe
physiological function
Pmt1 provides in vivo tRNA methylation activity that is strongly controlled by nutritional cues
Schizosaccharomyces pombe
Expression (protein specific)
Organism
Commentary
Expression
Schizosaccharomyces pombe
cultivation of Schizosaccharomyces pombe in minimal medium leads to approximately 23% of tRNAAsp methylation
down
Schizosaccharomyces pombe
methylation is not enhanced by stress conditions such as glucose starvation, heat stress or oxidative stress
additional information
Schizosaccharomyces pombe
tRNAAsp methylation is induced to 100% when cells are grown in the presence of peptone
up
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
-
1
-
13
-
-
-
-
1
-
1
-
1
-
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1
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1
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1
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1
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1
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1
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1
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13
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1
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1
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1
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1
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1
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1
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2
2
-
-
-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
-
-
1
-
-
-
-
-
3
-
-
6
-
3
-
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9
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6
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3
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1
3
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6
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9
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6
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-
-
-
9
9
-
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735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
-
-
-
-
-
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1
-
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2
-
14
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2
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1
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2
-
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-
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3
3
-
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-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
-
-
-
-
8
-
2
-
-
-
-
-
8
-
-
10
-
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1
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1
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1
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8
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8
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-
10
-
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-
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-
-
-
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2
2
-
-
-
736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
-
-
-
2
-
4
-
2
-
-
-
-
-
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7
-
2
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2
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2
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2
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1
2
-
2
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2
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4
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7
-
2
-
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2
-
-
-
3
2
2
3
-
-
736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
-
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-
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1
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
-
-
2
-
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4
-
1
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1
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1
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5
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1
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1
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5
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1
1
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718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
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1
-
24
-
-
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1
-
1
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2
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1
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3
1
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1
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1
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1
1
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24
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1
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1
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1
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3
1
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1
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1
1
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708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
-
-
-
-
-
-
-
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1
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1
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1
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5
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2
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1
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5
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2
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1
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2
2
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710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
-
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2
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1
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9
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
-
1
-
5
-
-
-
-
1
-
-
-
2
-
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1
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1
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1
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1
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5
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1
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1
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1
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1
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-
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-
-
-
-
708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
-
-
-
-
-
-
-
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1
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1
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1
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1
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-
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2
2
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710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
-
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1
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1
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1
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1
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1
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1
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1
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2
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