BRENDA - Enzyme Database show
show all sequences of 2.1.1.204

Identification of direct targets and modified bases of RNA cytosine methyltransferases

Khoddami, V.; Cairns, B.R.; Nat. Biotechnol. 31, 458-464 (2013)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5-aza-2'-deoxycytidine
the catalytic mechanism is disrupted by the suicide inhibitor
Homo sapiens
5-azacytidine
the catalytic mechanism is disrupted by the suicide inhibitor
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + cytosine38 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
the enzyme forms a covalent enzyme-substrate intermediate with its target
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal
736856
Homo sapiens
?
-
-
-
-
additional information
development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC) bears one DNMT2 target site (C38)
736856
Homo sapiens
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-aza-2'-deoxycytidine
the catalytic mechanism is disrupted by the suicide inhibitor
Homo sapiens
5-azacytidine
the catalytic mechanism is disrupted by the suicide inhibitor
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal
736856
Homo sapiens
?
-
-
-
-
additional information
development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC) bears one DNMT2 target site (C38)
736856
Homo sapiens
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
-
736856
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the DNMT2 family of cytosine 5-methylation-RNA methyltransferases utilizing only one cysteine in their catalytic pocket
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the DNMT2 family of cytosine 5-methylation-RNA methyltransferases utilizing only one cysteine in their catalytic pocket
Homo sapiens
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
-
1
-
13
-
-
-
-
1
-
1
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1
-
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1
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1
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1
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1
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1
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1
1
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13
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1
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1
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1
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1
-
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1
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1
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1
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2
2
-
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-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
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1
-
-
-
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3
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6
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3
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9
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6
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3
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1
3
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3
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6
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9
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6
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9
9
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735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
-
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1
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2
-
14
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2
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1
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1
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1
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2
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2
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-
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3
3
-
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-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
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-
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-
8
-
2
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8
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10
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1
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1
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1
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8
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8
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10
-
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2
2
-
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736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
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2
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4
-
2
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7
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2
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2
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2
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2
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1
2
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2
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2
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4
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7
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2
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2
-
-
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3
2
2
3
-
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736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
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2
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1
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5
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1
1
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718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
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1
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24
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1
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1
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2
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1
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3
1
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1
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1
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1
1
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24
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1
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1
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1
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3
1
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1
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1
1
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708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
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1
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1
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1
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1
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5
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2
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1
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2
2
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710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
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2
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1
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9
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
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1
-
5
-
-
-
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1
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2
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1
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1
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1
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1
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5
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1
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1
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1
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708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
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-
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1
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2
2
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710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
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1
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