BRENDA - Enzyme Database show
show all sequences of 2.1.1.204

The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis

Tuorto, F.; Herbst, F.; Alerasool, N.; Bender, S.; Popp, O.; Federico, G.; Reitter, S.; Liebers, R.; Stoecklin, G.; Groene, H.J.; Dittmar, G.; Glimm, H.; Lyko, F.; EMBO J. 34, 2350-2362 (2015)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of Dnmt2/- knockout mice
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
?
-
-
-
additional information
Mus musculus C57BL/6
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Mus musculus
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Mus musculus C57BL/6
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
O55055
-
-
Mus musculus C57BL/6
O55055
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adipocyte
-
Mus musculus
-
bone marrow cell
primary
Mus musculus
-
fibroblast
-
Mus musculus
-
haematopoietic tissue
-
Mus musculus
-
hematopoietic progenitor cell
Lin(neg) Sca1+cKit+ haematopoietic progenitor cells that represent a heterogeneous cell population containing the most primitive haematopoietic cells that have self-renewal capacity and haematopoietic progenitors that give rise to all mature cell types found in peripheral blood for a lifetime
Mus musculus
-
mesenchymal stromal cell
-
Mus musculus
-
additional information
enzyme Dnmt2 is expressed in various mouse tissues
Mus musculus
-
osteoblast
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
736018
Mus musculus
?
-
-
-
-
additional information
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
736018
Mus musculus C57BL/6
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of Dnmt2/- knockout mice
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
?
-
-
-
additional information
Mus musculus C57BL/6
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
?
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Mus musculus
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Mus musculus C57BL/6
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
Mus musculus
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
Mus musculus C57BL/6
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adipocyte
-
Mus musculus
-
bone marrow cell
primary
Mus musculus
-
fibroblast
-
Mus musculus
-
haematopoietic tissue
-
Mus musculus
-
hematopoietic progenitor cell
Lin(neg) Sca1+cKit+ haematopoietic progenitor cells that represent a heterogeneous cell population containing the most primitive haematopoietic cells that have self-renewal capacity and haematopoietic progenitors that give rise to all mature cell types found in peripheral blood for a lifetime
Mus musculus
-
mesenchymal stromal cell
-
Mus musculus
-
additional information
enzyme Dnmt2 is expressed in various mouse tissues
Mus musculus
-
osteoblast
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
736018
Mus musculus
?
-
-
-
-
additional information
Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
736018
Mus musculus C57BL/6
?
-
-
-
-
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
736018
Mus musculus
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
-
736018
Mus musculus C57BL/6
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
specific codon mistranslation by tRNAs lacking Dnmt2-dependent methylation causes systematic differences in protein expression, with 153 significantly deregulated proteins among a total of 4094 identified proteins. Enzyme-deficient Dnmt2-/- mice reveal delayed endochondral ossification of the long bones with uantitative differences in the length of the zone of cell maturation and hypertrophy in the epiphyseal plate between wild-type and Dnmt2-/- mice as well as in the length of the trabecular zone between wild-type and Dnmt2-/- mice. The trabecular structures are not only reduced in length but also appear incorrectly interfaced with bone marrow cells. The numbers of capillaries counted in the trabecular zone are significantly reduced. Enzyme-deficient bone marrow cells show an enlarged pool of osteo-progenitors. Haematopoietic phenotype, detailed overview
Mus musculus
physiological function
Dnmt2 plays an important role in haematopoiesis and define an additional function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis, role for Dnmt2 in the regulation of codon fidelity. The enzyme prevents tRNA fragmentation. Enzyme Dnmt2 is required for cell differentiation, e.g. of bone marrow mesenchymal stromal cells and for cell-autonomous differentiation during haematopoiesis
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
specific codon mistranslation by tRNAs lacking Dnmt2-dependent methylation causes systematic differences in protein expression, with 153 significantly deregulated proteins among a total of 4094 identified proteins. Enzyme-deficient Dnmt2-/- mice reveal delayed endochondral ossification of the long bones with uantitative differences in the length of the zone of cell maturation and hypertrophy in the epiphyseal plate between wild-type and Dnmt2-/- mice as well as in the length of the trabecular zone between wild-type and Dnmt2-/- mice. The trabecular structures are not only reduced in length but also appear incorrectly interfaced with bone marrow cells. The numbers of capillaries counted in the trabecular zone are significantly reduced. Enzyme-deficient bone marrow cells show an enlarged pool of osteo-progenitors. Haematopoietic phenotype, detailed overview
Mus musculus
physiological function
Dnmt2 plays an important role in haematopoiesis and define an additional function of C38 tRNA methylation in the discrimination of near-cognate codons, thereby ensuring accurate polypeptide synthesis, role for Dnmt2 in the regulation of codon fidelity. The enzyme prevents tRNA fragmentation. Enzyme Dnmt2 is required for cell differentiation, e.g. of bone marrow mesenchymal stromal cells and for cell-autonomous differentiation during haematopoiesis
Mus musculus
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
-
1
-
13
-
-
-
-
1
-
1
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1
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1
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1
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1
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1
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1
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1
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1
1
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13
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1
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1
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1
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1
-
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1
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1
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1
-
-
-
-
2
2
-
-
-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
-
-
1
-
-
-
-
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3
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6
-
3
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9
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6
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3
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1
3
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3
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6
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9
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6
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9
9
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735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
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-
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1
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2
-
14
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2
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1
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1
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1
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2
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2
-
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3
3
-
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-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
-
-
-
-
8
-
2
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8
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10
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1
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1
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1
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8
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8
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-
10
-
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-
-
-
-
-
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2
2
-
-
-
736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
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-
2
-
4
-
2
-
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7
-
2
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-
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2
-
-
2
-
-
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2
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1
2
-
2
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2
-
4
-
-
-
-
-
-
-
-
7
-
2
-
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-
2
-
-
-
3
2
2
3
-
-
736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
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1
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
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2
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4
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1
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1
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1
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5
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1
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1
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4
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1
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5
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1
1
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718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
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1
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24
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1
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1
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2
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1
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3
1
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1
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1
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1
1
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24
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1
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1
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1
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3
1
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1
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1
1
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708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
-
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-
-
-
-
-
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1
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1
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1
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5
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2
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1
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1
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1
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5
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2
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1
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2
2
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710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
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2
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1
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9
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2
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
-
1
-
5
-
-
-
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1
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2
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1
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1
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1
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1
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5
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1
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1
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1
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1
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708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
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-
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1
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1
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1
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1
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1
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710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
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