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show all sequences of 2.1.1.204

Somatic cancer mutations in the DNMT2 tRNA methyltransferase alter its catalytic properties

Elhardt, W.; Shanmugam, R.; Jurkowski, T.P.; Jeltsch, A.; Biochimie 112, 66-72 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
D217H
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
D226Y
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
D255Y
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E185K
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E202Q
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E317G
site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E63K
site-directed mutagenesis, the mutation causes a twofold increase in activity compared to the wild-type enzyme
Homo sapiens
G155S
site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme
Homo sapiens
G155V
site-directed mutagenesis, almost inactive mutant
Homo sapiens
L257V
site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme
Homo sapiens
M72I
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
N264S
site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
R371H
site-directed mutagenesis, almost inactive mutant
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
Dnmt2 is consistently upregulated in hundreds of tumor samples
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
735726
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D217H
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
D226Y
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
D255Y
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E185K
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E202Q
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E317G
site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
E63K
site-directed mutagenesis, the mutation causes a twofold increase in activity compared to the wild-type enzyme
Homo sapiens
G155S
site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme
Homo sapiens
G155V
site-directed mutagenesis, almost inactive mutant
Homo sapiens
L257V
site-directed mutagenesis, the mutation causes an over fourfold decrease in activity compared to the wild-type enzyme
Homo sapiens
M72I
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
N264S
site-directed mutagenesi, the mutant shows activity similar to the wild-type enzyme
Homo sapiens
R371H
site-directed mutagenesis, almost inactive mutant
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
Dnmt2 is consistently upregulated in hundreds of tumor samples
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
735726
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
evolution
the enzyme is a highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNA-Asp in many species, including lower eukaryotes, plants, insects and humans
Homo sapiens
physiological function
The DNMT2 protein methylates C38 of tRNA-Asp and it has a role in cellular physiology and stress response and its expression levels are altered in cancer tissues
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNA-Asp in many species, including lower eukaryotes, plants, insects and humans
Homo sapiens
physiological function
The DNMT2 protein methylates C38 of tRNA-Asp and it has a role in cellular physiology and stress response and its expression levels are altered in cancer tissues
Homo sapiens
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
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1
-
13
-
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
-
1
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1
-
-
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1
-
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1
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1
1
-
13
-
-
-
-
-
-
1
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1
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-
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1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
-
-
1
-
-
-
-
-
3
-
-
6
-
3
-
-
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-
9
-
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6
-
-
-
-
-
-
-
-
3
-
-
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1
3
-
-
-
-
-
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3
-
-
6
-
-
-
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9
-
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6
-
-
-
-
-
-
-
-
-
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9
9
-
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735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
-
-
-
-
-
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-
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1
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2
-
14
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2
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1
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1
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1
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2
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2
-
-
-
-
-
-
-
-
-
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3
3
-
-
-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
-
-
-
-
8
-
2
-
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8
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10
-
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1
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-
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1
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1
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8
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8
-
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10
-
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-
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-
-
-
-
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2
2
-
-
-
736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
-
-
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2
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4
-
2
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-
-
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7
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2
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2
-
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2
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2
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1
2
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2
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2
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4
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7
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2
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2
-
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3
2
2
3
-
-
736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
-
-
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1
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
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1
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1
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5
-
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1
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1
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2
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4
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1
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5
-
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-
-
-
-
-
-
1
1
-
-
-
718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
-
1
-
24
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
3
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
24
-
-
-
-
-
-
1
-
1
-
-
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1
-
-
-
-
3
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
-
-
-
-
-
-
-
-
1
-
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1
-
1
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-
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5
-
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2
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1
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1
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1
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5
-
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2
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1
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-
-
-
-
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2
2
-
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-
710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
-
-
-
-
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-
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2
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1
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9
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2
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
-
1
-
5
-
-
-
-
1
-
-
-
2
-
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1
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-
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1
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1
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1
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5
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1
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1
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1
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1
-
-
-
-
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-
-
-
-
708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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2
2
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710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
-
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1
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1
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1
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1
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2
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1
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1
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1
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2
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