BRENDA - Enzyme Database show
show all sequences of 2.1.1.204

Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2

Goll, M.G.; Kirpekar, F.; Maggert, K.A.; Yoder, J.A.; Hsieh, C.L.; Zhang, X.; Golic, K.G.; Jacobsen, S.E.; Bestor, T.H.; Science 311, 395-398 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in NIH3T3 cells
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
human DNMT2 protein (hDNMT2) is primarily localized to the cytoplasm of transfected mouse 3T3 fibroblasts
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
O14717
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
-
710560
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
methylates tRNAAsp specifically at cytosine38 in the anticodon loop. Human DNMT2 protein restores methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that is dependent on preexisting patterns of modified nucleosides. Unmodified tRNAAsp produced by in vitro transcription is not a substrate for DNMT2, which suggests that methylation is guided to cytosine38 by other modifications. Mannosylqueuosine is likely to be involved, because it is unique to tRNAAsp. Analysis of tRNAAsp sequences show complete conservation of the anticodon loop in species whose genomes encode Dnmt2 homologs, but the tRNAAsp anticodon loops in Caenorhabditis elegans and Saccharomyces cerevisiae, which lack Dnmt2 homologs, have diverged
710560
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in NIH3T3 cells
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
human DNMT2 protein (hDNMT2) is primarily localized to the cytoplasm of transfected mouse 3T3 fibroblasts
Homo sapiens
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
-
710560
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
methylates tRNAAsp specifically at cytosine38 in the anticodon loop. Human DNMT2 protein restores methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that is dependent on preexisting patterns of modified nucleosides. Unmodified tRNAAsp produced by in vitro transcription is not a substrate for DNMT2, which suggests that methylation is guided to cytosine38 by other modifications. Mannosylqueuosine is likely to be involved, because it is unique to tRNAAsp. Analysis of tRNAAsp sequences show complete conservation of the anticodon loop in species whose genomes encode Dnmt2 homologs, but the tRNAAsp anticodon loops in Caenorhabditis elegans and Saccharomyces cerevisiae, which lack Dnmt2 homologs, have diverged
710560
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
-
1
-
13
-
-
-
-
1
-
1
-
1
-
-
1
-
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1
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1
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1
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1
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1
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1
1
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13
-
-
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1
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1
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1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
-
-
1
-
-
-
-
-
3
-
-
6
-
3
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-
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-
-
9
-
-
6
-
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-
-
-
-
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-
3
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1
3
-
-
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3
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6
-
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9
-
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6
-
-
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-
-
-
-
-
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9
9
-
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-
735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
-
-
-
-
-
-
-
-
1
-
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2
-
14
-
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-
-
-
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2
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-
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1
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1
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1
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2
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-
2
-
-
-
-
-
-
-
-
-
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3
3
-
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-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
-
-
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8
-
2
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8
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10
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1
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1
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1
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8
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8
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10
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2
2
-
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-
736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
-
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2
-
4
-
2
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7
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2
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2
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2
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2
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1
2
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2
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2
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4
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7
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2
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2
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3
2
2
3
-
-
736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
-
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1
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
-
-
2
-
-
-
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4
-
1
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1
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1
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5
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1
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1
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2
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4
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1
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5
-
-
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-
-
-
-
-
-
1
1
-
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-
718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
-
1
-
24
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
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3
1
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1
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1
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1
1
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24
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1
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1
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1
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3
1
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1
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1
1
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708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
-
-
-
-
-
-
-
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1
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1
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1
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5
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2
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1
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1
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1
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5
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2
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1
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2
2
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710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
-
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2
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1
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9
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2
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
-
1
-
5
-
-
-
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1
-
-
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2
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1
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1
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1
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1
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5
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1
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1
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1
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1
-
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708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
-
-
-
-
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1
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1
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1
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1
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1
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2
2
-
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-
710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
-
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1
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1
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1
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1
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2
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1
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