BRENDA - Enzyme Database show
show all sequences of 2.1.1.204

Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism

Jurkowski, T.P.; Meusburger, M.; Phalke, S.; Helm, M.; Nellen, W.; Reuter, G.; Jeltsch, A.; RNA 14, 1663-1670 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C292A
about 3fold reduction in RNA binding affinity. Significant residual activity
Homo sapiens
C79A
about 3fold reduction in RNA binding affinity. No detectable in vitro methylation activity
Homo sapiens
E119A
mutant binds stronger to RNA than wild-type. No detectable in vitro methylation activity
Homo sapiens
R160A
no detectable in vitro methylation activity
Homo sapiens
R162A
no detectable in vitro methylation activity
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
supports native folding of the substrate tRNA
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
in vitro transcribed tRNAAsp is methylated by DNMT2, albeit at reduced efficiency. Human, mouse, and Dictyostelium tRNA all are substrates for human DNMT2. C79, E119, R160 and R162 are essential for the catalytic mechanism of DNMT2
710547
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C292A
about 3fold reduction in RNA binding affinity. Significant residual activity
Homo sapiens
C79A
about 3fold reduction in RNA binding affinity. No detectable in vitro methylation activity
Homo sapiens
E119A
mutant binds stronger to RNA than wild-type. No detectable in vitro methylation activity
Homo sapiens
R160A
no detectable in vitro methylation activity
Homo sapiens
R162A
no detectable in vitro methylation activity
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
supports native folding of the substrate tRNA
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine38 in tRNA
in vitro transcribed tRNAAsp is methylated by DNMT2, albeit at reduced efficiency. Human, mouse, and Dictyostelium tRNA all are substrates for human DNMT2. C79, E119, R160 and R162 are essential for the catalytic mechanism of DNMT2
710547
Homo sapiens
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Other publictions for EC 2.1.1.204
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735726
Elhardt
Somatic cancer mutations in th ...
Homo sapiens
Biochimie
112
66-72
2015
-
-
1
-
13
-
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
-
1
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1
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1
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1
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-
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1
1
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13
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1
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1
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1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735727
Uysal
Dynamic expression of DNA meth ...
Bos taurus, Homo sapiens, Mus musculus
Biochimie
116
103-113
2015
-
-
1
-
-
-
-
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3
-
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6
-
3
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9
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6
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3
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1
3
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3
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6
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9
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6
-
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-
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-
-
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-
-
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9
9
-
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-
735864
Burgess
Conservation of tRNA and rRNA ...
Arabidopsis thaliana
BMC Plant Biol.
15
199
2015
-
-
-
-
-
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-
1
-
-
2
-
14
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2
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1
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1
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2
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2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
736018
Tuorto
The tRNA methyltransferase Dnm ...
Mus musculus, Mus musculus C57BL/6
EMBO J.
34
2350-2362
2015
-
-
-
-
1
-
-
-
-
-
-
8
-
2
-
-
-
-
-
8
-
-
10
-
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-
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1
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-
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-
1
-
1
-
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8
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8
-
-
10
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
736905
Mueller
Dynamic modulation of Dnmt2-de ...
Dictyostelium discoideum, Schizosaccharomyces pombe
Nucleic Acids Res.
43
10952-10962
2015
2
-
1
-
2
-
-
-
-
2
-
4
-
2
-
-
-
-
-
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7
-
2
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-
2
-
-
2
-
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2
-
1
2
-
2
-
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-
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2
-
4
-
-
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-
-
-
-
-
7
-
2
-
-
-
2
-
-
-
3
2
2
3
-
-
736775
Militello
A map of 5-methylcytosine resi ...
no activity in Trypanosoma brucei
Mol. Biochem. Parasitol.
193
122-126
2014
-
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1
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736856
Khoddami
Identification of direct targe ...
Homo sapiens
Nat. Biotechnol.
31
458-464
2013
-
-
-
-
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2
-
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4
-
1
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1
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1
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5
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1
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1
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2
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4
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1
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5
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1
1
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718931
Jurkowski
Mapping the tRNA binding site ...
Homo sapiens
Biochemistry
51
4438-4444
2012
-
-
1
-
24
-
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-
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1
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1
-
2
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1
-
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3
1
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1
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1
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1
1
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24
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1
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1
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1
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3
1
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1
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1
1
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708588
Schaefer
RNA methylation by Dnmt2 prote ...
Drosophila melanogaster
Genes Dev.
24
1590-1595
2010
-
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1
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1
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1
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5
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2
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1
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5
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2
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1
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2
2
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710349
Schaefer
The Drosophila cytosine-5 meth ...
Drosophila melanogaster
PLoS One
3
e1414
2008
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2
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1
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9
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710547
Jurkowski
Human DNMT2 methylates tRNA(As ...
Drosophila melanogaster, Homo sapiens
RNA
14
1663-1670
2008
-
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1
-
5
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1
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2
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1
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1
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5
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1
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1
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708586
Rai
Dnmt2 functions in the cytopla ...
Danio rerio
Genes Dev.
21
261-266
2007
-
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-
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1
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1
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2
2
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710560
Goll
Methylation of tRNAAsp by the ...
Homo sapiens
Science
311
395-398
2006
-
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1
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1
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1
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1
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