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Literature summary for 2.1.1.203 extracted from

  • Genenncher, B.; Durdevic, Z.; Hanna, K.; Zinkl, D.; Mobin, M.B.; Senturk, N.; Da Silva, B.; Legrand, C.; Carre, C.; Lyko, F.; Schaefer, M.
    Mutations in cytosine-5 tRNA methyltransferases impact mobile element expression and genome stability at specific DNA repeats (2018), Cell Rep., 22, 1861-1874 .
    View publication on PubMed
Search for more literature for 2.1.1.203

Cloned(Commentary)

Cloned (Comment) Organism
gene NSun2, recombinant expression of wild-type and mutant enzymes Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information generation of an insertion mutation in Drosophila NSun2 (NSun2DELTA21.5), which is viable and fertile and loses RNA methylation at known tRNA substrates. RNAi-mediated knockdown of NSun2 in follicle cells. Functional Gypsy retroviral particles can be formed in NSun2 mutants. Specific eccDNAs accumulate in RCMT mutants. RCMT mutants display reduced tRNA stability and tRNA abundance Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine34 in tRNA precursor Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9W4M9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
ovarian follicle
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytosine34 in tRNA precursor
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?

Synonyms

Synonyms Comment Organism
cytosine-5 tRNA methyltransferase
-
 Drosophila melanogaster
NSUN2
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 Drosophila melanogaster
RCMT
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction mutation in (cytosine-5) RNA methyltransferase NSun2, which targets mostly tRNAs, impacts the expression of mobile element-derived sequences and affects DNA repeat integrity in Drosophila melanogaster. Reduced tRNA stability in the RCMT mutant indicates that tRNA-dependent processes affect mobile element expression and DNA repeat stability. NSun2 function affects mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in both RCMT mutants indicates that tRNA-dependent processes affect mobile element expression and DNA repeat stability. NSun2 mutants show heat-shock-independent transposable element (TE) expression changes Drosophila melanogaster
physiological function mutations in two (cytosine-5) RNA methyltransferase NSun2 impact the accumulation of mobile element-derived sequences and DNA repeat integrity in Drosophila melanogaster. NSun2 function affects mobile element expression and genome integrity in a heat shock-independent fashion. Reduced tRNA stability in mutants indicates that tRNA-dependent processes affected mobile element expression and DNA repeat stability Drosophila melanogaster
physiological function NSun2 protein is a highly conserved (cytosine-5) methyltransferases that methylate specific tRNAs instead of genomic DNA. NSun2 function affects repeat elements in a heat shock-independent fashion. NSun2, is an RCMT targeting the majority of tRNAs Drosophila melanogaster