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Literature summary for 2.1.1.202 extracted from

  • Khoddami, V.; Cairns, B.R.
    Identification of direct targets and modified bases of RNA cytosine methyltransferases (2013), Nat. Biotechnol., 31, 458-464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.202

Protein Variants

Protein Variants Comment Organism
additional information siRNA-mediated enzyme knockout in HeLa cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-aza-2'-deoxycytidine the catalytic mechanism is disrupted by the suicide inhibitor Homo sapiens
5-azacytidine the catalytic mechanism is disrupted by the suicide inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleolus
-
 Homo sapiens 5730
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides ?
-
 ?
S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNALeu precursor
-
 ?
S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly precursor
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor the enzyme forms a covalent enzyme-substrate intermediate with its target Homo sapiens
a
S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor the enzyme forms a covalent enzyme-substrate intermediate with its target Homo sapiens
a
S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA the enzyme forms a covalent enzyme-substrate intermediate with its target Homo sapiens
a
S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA the enzyme forms a covalent enzyme-substrate intermediate with its target Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides Homo sapiens ?
-
 ?
additional information development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. The method enables over 200fold enrichment of tRNAs that are known targets of the enzyme revealing many tRNA and non-coding RNA targets not previously associated with NSUN2. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC), bears four NSUN2 target sites (C40, 48, 49 and 50) Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNALeu precursor
-
 ?
S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp precursor
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly precursor
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly precursor
-
 ?

Synonyms

Synonyms Comment Organism
NSUN2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the RsmF/YebU/NSUN2 family of cytosine 5-methylation-RNA methyltransferases utilizing two cysteines in their catalytic pocket Homo sapiens
malfunction NSUN2 is associated with Myc-induced proliferation of cancer cells, mitotic spindle stability, infertility in male mice, and the balance of selfrenewal and differentiation in skin stem cells. In humans NSUN2 mutations cause an autosomal recessive syndrome characterized by intellectual disability and mental retardation Homo sapiens