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show all sequences of 2.1.1.201

Molecular characterization of the human COQ5 C-methyltransferase in coenzyme Q10 biosynthesis

Nguyen, T.P.; Casarin, A.; Desbats, M.A.; Doimo, M.; Trevisson, E.; Santos-Ocana, C.; Navas, P.; Clarke, C.F.; Salviati, L.; Biochim. Biophys. Acta 1841, 1628-1638 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the gene encoding human COQ5 spans to 26 kb on chromosome 12q24.31 and is comprised of seven exons, HEK-293 cells are co-transfected with the pCOQ5-myc and pCOQ4-V5, expression of a COQ5-GFP construct in HeLa cells, construction of a hybrid yeast-human COQ5 (yhCOQ5) gene by amplifying a 5' segment of yCOQ5, corresponding to the mitochondrial targeting region (encoding aa 1-54) and the 3' of human COQ5
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
additional information
complementation of yeast coq5 point and null mutants by expression of the human enzyme. Stabilization of the CoQ synthome within coq5 point mutants or by overexpression of COQ8 in coq5 null mutants permits the human COQ5 homologue to partially restore coq5 mutant growth on respiratory media and Q6 content. Human and Escherichia coli Coq5 homologues expressed in yeast retain C-methyltransferase activity but are capable of rescuing the coq5 yeast mutants only when the CoQ-synthome is assembled
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
the COQ5 polypeptide is associated with the mitochondrial inner membrane on the matrix side
Homo sapiens
5739
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q5HYK3
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
human COQ5 RNA is expressed in all tissues
Homo sapiens
-
skin fibroblast
-
Homo sapiens
-
Cloned(Commentary) (protein specific)
Commentary
Organism
the gene encoding human COQ5 spans to 26 kb on chromosome 12q24.31 and is comprised of seven exons, HEK-293 cells are co-transfected with the pCOQ5-myc and pCOQ4-V5, expression of a COQ5-GFP construct in HeLa cells, construction of a hybrid yeast-human COQ5 (yhCOQ5) gene by amplifying a 5' segment of yCOQ5, corresponding to the mitochondrial targeting region (encoding aa 1-54) and the 3' of human COQ5
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
complementation of yeast coq5 point and null mutants by expression of the human enzyme. Stabilization of the CoQ synthome within coq5 point mutants or by overexpression of COQ8 in coq5 null mutants permits the human COQ5 homologue to partially restore coq5 mutant growth on respiratory media and Q6 content. Human and Escherichia coli Coq5 homologues expressed in yeast retain C-methyltransferase activity but are capable of rescuing the coq5 yeast mutants only when the CoQ-synthome is assembled
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
the COQ5 polypeptide is associated with the mitochondrial inner membrane on the matrix side
Homo sapiens
5739
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
human COQ5 RNA is expressed in all tissues
Homo sapiens
-
skin fibroblast
-
Homo sapiens
-
General Information
General Information
Commentary
Organism
malfunction
in humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in coenzyme Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases
Homo sapiens
physiological function
enzyme Coq5 catalyzes the only C-methylation involved in the biosynthesis of coenzymeQ(Q or ubiquinone) in humans
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
in humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in coenzyme Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases
Homo sapiens
physiological function
enzyme Coq5 catalyzes the only C-methylation involved in the biosynthesis of coenzymeQ(Q or ubiquinone) in humans
Homo sapiens
Other publictions for EC 2.1.1.201
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735364
Dai
Crystal structures and catalyt ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
70
2085-2092
2014
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735704
Nguyen
Molecular characterization of ...
Homo sapiens
Biochim. Biophys. Acta
1841
1628-1638
2014
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720768
Zhou
Think outside the box: seleniu ...
Brassica oleracea
Plant Signal. Behav.
5
76-77
2010
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706320
Zhou
Involvement of a broccoli COQ5 ...
Brassica oleracea
Plant Physiol.
151
528-540
2009
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704408
Baba
Yeast Coq5 C-methyltransferase ...
Saccharomyces cerevisiae
J. Biol. Chem.
279
10052-10059
2004
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702398
Hagerman
The yeast gene COQ5 is differe ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1578
51-58
2002
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3
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703812
Hagerman
The regulation of COQ5 gene ex ...
Saccharomyces cerevisiae
Free Radic. Res.
36
485-490
2002
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698549
Lee
A C-methyltransferase involved ...
Escherichia coli
J. Bacteriol.
179
1748-1754
1997
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704387
Dibrov
The COQ5 gene encodes a yeast ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
9175-9181
1997
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704388
Barkovich
Characterization of the COQ5 g ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
9182-918
1997
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704239
Young
Characterization and genetic a ...
Escherichia coli K-12
J. Bacteriol.
105
769-778
1971
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