Literature summary for 2.1.1.20 extracted from

Luka, Z.; Pakhomova, S.; Loukachevitch, L.V.; Egli, M.; Newcomer, M.E.; Wagner, C.
5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase (2007), J. Biol. Chem., 282, 4069-4075.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Rattus norvegicus
expressed in Escherichia coli BL21(GE3)	Rattus norvegicus
into pET-17b vector and expressed in Escherichia coli BL21(GE3)	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallized by the sitting drop method in complex with (6S)-5-methyltetrahydrofolat monoglutamate	Rattus norvegicus
GNMT complexed with 5-methyltetrahydrofolate, by the sitting drop method at room temperature, two folate binding sites in the intersubunit areas of the tetramer, each folate binding site is formed primarily by two 1-7 N-terminal regions of one pair of subunits and two 205-218 regions of the other pair of subunits. Both the pteridine and p-aminobenzoyl rings are located in the hydrophobic cavities formed by Tyr5, Leu207, and Met215 residues of all subunits	Rattus norvegicus
sitting-drop vapor diffusion method in complex with 5-methyltetrahydrofolate pentaglutamate, two molecules of inhibitor bound to a tetramer	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
5-methyltetrahydrofolate	-	Rattus norvegicus
5-Methyltetrahydrofolate pentaglutamate	-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Rattus norvegicus	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	major folate binding protein	?	-	?
S-adenosyl-L-methionine + glycine	Rattus norvegicus	key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio	S-adenosyl-L-homocysteine + N-methylglycine	-	?
S-adenosyl-L-methionine + glycine	Rattus norvegicus	key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell	S-adenosyl-L-homocysteine + N-methylglycine	N-methylglycine = sarcosine	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P13255	-	-

Purification (Commentary)

Purification (Comment)	Organism
by ammonium sulfate precipitation and gel filtration	Rattus norvegicus
recombinant protein	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	major folate binding protein	Rattus norvegicus	?	-	?
S-adenosyl-L-methionine + glycine	key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio	Rattus norvegicus	S-adenosyl-L-homocysteine + N-methylglycine	-	?
S-adenosyl-L-methionine + glycine	key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell	Rattus norvegicus	S-adenosyl-L-homocysteine + N-methylglycine	N-methylglycine = sarcosine	?

Subunits

Subunits	Comment	Organism
tetramer	crystal structure analysis	Rattus norvegicus
tetramer	crystallographic studies	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
GNMT	-	Rattus norvegicus

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Release 2023.1 (January 2023)