Literature summary for 2.1.1.2 extracted from

Im, Y.S.; Chiang, P.K.; Cantoni, G.L.
Guanidoacetate methyltransferase. Purification and molecular properties (1979), J. Biol. Chem., 254, 11047-11050.

Search for more literature for 2.1.1.2

Inhibitors

Inhibitors	Comment	Organism	Structure
3-deaza-S-adenosyl-L-homocysteine	competitive with S-adenosylmethionine	Sus scrofa
S-adenosyl-L-homocysteine	competitive with S-adenosylmethionine	Sus scrofa
sinefungin	competitive with S-adenosylmethionine	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	Guanidinoacetate	-	Sus scrofa
0.049	-	S-adenosyl-L-methionine	-	Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	SDS-PAGE, native PAGE without SDS yields heterogeneity in molecular weight	Sus scrofa
31500	-	gel filtration	Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanidinoacetate	Sus scrofa	-	S-adenosyl-L-homocysteine + creatine	-	?

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
27.2	-	purified enzyme	Sus scrofa

Storage Stability

Storage Stability	Organism
-60°C, purified enzyme, stable for months	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanidinoacetate	-	Sus scrofa	S-adenosyl-L-homocysteine + creatine	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.016	-	adenosylhomocysteine	-	Sus scrofa
0.018	-	sinefungin	-	Sus scrofa
0.039	-	3-deaza-S-adenosyl-L-homocysteine	-	Sus scrofa

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Release 2023.1 (January 2023)