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Literature summary for 2.1.1.2 extracted from
- Enzymatic mechanism of creatine synthesis (1954), J. Biol. Chem., 209, 647-659.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CN- | - |
Cavia porcellus |  |
| CN- | - |
Sus scrofa | |
| CN- | - |
Bos taurus | |
| CN- | - |
Oryctolagus cuniculus | |
| cysteine | - |
Cavia porcellus | |
| cysteine | - |
Sus scrofa | |
| cysteine | - |
Bos taurus | |
| cysteine | - |
Oryctolagus cuniculus | |
| glutathione | required for maximum activity, can be replaced by other reducing agents | Cavia porcellus | |
| glutathione | required for maximum activity, can be replaced by other reducing agents | Sus scrofa | |
| glutathione | required for maximum activity, can be replaced by other reducing agents | Bos taurus | |
| glutathione | required for maximum activity, can be replaced by other reducing agents | Oryctolagus cuniculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | complete inhibition at 1 mM, reversible by addition of glutathione | Bos taurus | |
| p-chloromercuribenzoate | complete inhibition at 1 mM, reversible by addition of glutathione | Cavia porcellus | |
| p-chloromercuribenzoate | complete inhibition at 1 mM, reversible by addition of glutathione | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | complete inhibition at 1 mM, reversible by addition of glutathione | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Cavia porcellus | |
| Mg2+ | - |
Sus scrofa | |
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanidinoacetate | Cavia porcellus | biosynthesis of creatine | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | Sus scrofa | biosynthesis of creatine | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | Bos taurus | biosynthesis of creatine | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | Oryctolagus cuniculus | biosynthesis of creatine | S-adenosyl-L-homocysteine + creatine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.022 | - |
purified enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanidinoacetate | S-adenosyl-L-methionine is the only possible methyl donor | Cavia porcellus | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | S-adenosyl-L-methionine is the only possible methyl donor | Sus scrofa | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | S-adenosyl-L-methionine is the only possible methyl donor | Bos taurus | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | S-adenosyl-L-methionine is the only possible methyl donor | Oryctolagus cuniculus | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | biosynthesis of creatine | Cavia porcellus | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | biosynthesis of creatine | Sus scrofa | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | biosynthesis of creatine | Bos taurus | S-adenosyl-L-homocysteine + creatine | - |
? | |
| S-adenosyl-L-methionine + guanidinoacetate | biosynthesis of creatine | Oryctolagus cuniculus | S-adenosyl-L-homocysteine + creatine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Cavia porcellus |
| 7.5 | - |
- |
Sus scrofa |
| 7.5 | - |
- |
Bos taurus |
| 7.5 | - |
- |
Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cavia porcellus | |
| ATP | - |
Sus scrofa | |
| ATP | - |
Bos taurus | |
| ATP | - |
Oryctolagus cuniculus | |
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