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show all sequences of 2.1.1.193

The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis

Kumar, A.; Kumar, S.; Taneja, B.; Acta Crystallogr. Sect. D 70, 821-832 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24C
Mycobacterium tuberculosis
Engineering
Amino acid exchange
Commentary
Organism
additional information
the deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
Mycobacterium tuberculosis
-
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WGX1
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Mono S column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
-
733010
Mycobacterium tuberculosis
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
x-ray crystallography
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24C
Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
Mycobacterium tuberculosis
-
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Mono S column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
-
733010
Mycobacterium tuberculosis
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x-ray crystallography
Mycobacterium tuberculosis
Other publictions for EC 2.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733010
Kumar
The structure of Rv2372c ident ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
70
821-832
2014
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735238
Gutierrez
Indigenous and acquired modifi ...
Pseudomonas aeruginosa
RNA Biol.
10
1324-1332
2013
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720262
Zhang
Insights into the catalytic me ...
Escherichia coli
J. Mol. Biol.
423
576-589
2012
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3
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706717
Basturea
Substrate specificity and prop ...
Escherichia coli
RNA
13
1969-1976
2007
1
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706714
Basturea
Identification and characteriz ...
Escherichia coli
RNA
12
426-434
2006
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