Literature summary for 2.1.1.193 extracted from

Kumar, A.; Kumar, S.; Taneja, B.
The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis (2014), Acta Crystallogr. Sect. D, 70, 821-832.

Search for more literature for 2.1.1.193

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) and RsmE-deleted Escherichia coli KL16 cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.0, 1.5 M potassium acetate, at 24°C	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	the deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + uracil1498 in 16S rRNA	Mycobacterium tuberculosis	-	S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WGX1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Mono S column chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + uracil1498 in 16S rRNA	-	Mycobacterium tuberculosis	S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA	-	?

Subunits

Subunits	Comment	Organism
dimer	x-ray crystallography	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
RsmE	-	Mycobacterium tuberculosis
Rv2372c	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)