BRENDA - Enzyme Database show
show all sequences of 2.1.1.193

Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE

Basturea, G.N.; Deutscher, M.P.; RNA 13, 1969-1976 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
spermidine
in the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
10 mM, complete inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
uracil1498 in 16S rRNA
pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit
Escherichia coli
0.0267
-
S-adenosyl-L-methionine
pH 7.0, 37°C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
most active in the presence of 10–15 mM Mg2+ at pH 7–9. At 30 mM Mg2+ optimal activity is reduced by 60%. In the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM
Escherichia coli
NH4+
most active in the presence of 100 mM NH4Cl at pH 7–9
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
Escherichia coli
activity is dependent on a fully assembled 30 S ribosome subunit
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0AGL7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
activity is dependent on a fully assembled 30 S ribosome subunit
706717
Escherichia coli
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits are active. 16S rRNA obtained from the mutant strain, synthetic 16S rRNA, and 3' minor domain RNA are all very poor or inactive as substrates. 30S particles partially depleted of proteins by treatment with high concentrations of LiCl or in vitro reconstituted intermediate particles also show little or no methyl acceptor activity. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation
706717
Escherichia coli
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
forms dimers in solution
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
S-adenosyl-L-methionine
pH 7.0, 37°C
Escherichia coli
0.0013
-
uracil1498 in 16S rRNA
pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
spermidine
in the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
10 mM, complete inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
uracil1498 in 16S rRNA
pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit
Escherichia coli
0.0267
-
S-adenosyl-L-methionine
pH 7.0, 37°C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
most active in the presence of 10–15 mM Mg2+ at pH 7–9. At 30 mM Mg2+ optimal activity is reduced by 60%. In the presence of spermidine, Mg2+ is not required for activity. Activity could be restored up to 60% of that with Mg2+ by addition of spermidine at 30 mM
Escherichia coli
NH4+
most active in the presence of 100 mM NH4Cl at pH 7–9
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
Escherichia coli
activity is dependent on a fully assembled 30 S ribosome subunit
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
activity is dependent on a fully assembled 30 S ribosome subunit
706717
Escherichia coli
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits are active. 16S rRNA obtained from the mutant strain, synthetic 16S rRNA, and 3' minor domain RNA are all very poor or inactive as substrates. 30S particles partially depleted of proteins by treatment with high concentrations of LiCl or in vitro reconstituted intermediate particles also show little or no methyl acceptor activity. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation
706717
Escherichia coli
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
forms dimers in solution
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0007
-
S-adenosyl-L-methionine
pH 7.0, 37°C
Escherichia coli
0.0013
-
uracil1498 in 16S rRNA
pH 7.0, 37°C, the Km-value for 16S rRNA is measured as a Km for the physiological substrate, 30S ribosomal subunit
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
Other publictions for EC 2.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733010
Kumar
The structure of Rv2372c ident ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
70
821-832
2014
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1
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735238
Gutierrez
Indigenous and acquired modifi ...
Pseudomonas aeruginosa
RNA Biol.
10
1324-1332
2013
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720262
Zhang
Insights into the catalytic me ...
Escherichia coli
J. Mol. Biol.
423
576-589
2012
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1
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2
1
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1
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3
3
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706717
Basturea
Substrate specificity and prop ...
Escherichia coli
RNA
13
1969-1976
2007
1
-
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1
2
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2
1
1
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1
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2
1
1
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1
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2
1
1
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2
1
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706714
Basturea
Identification and characteriz ...
Escherichia coli
RNA
12
426-434
2006
-
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1
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1
1
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3
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1
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