BRENDA - Enzyme Database show
show all sequences of 2.1.1.189

RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility

Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.; Nucleic Acids Res. 43, 8964-8972 (2015)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Streptococcus pneumoniae
the enzyme also methylates uracil1939 in 23S rRNA
?
-
-
-
additional information
Streptococcus pneumoniae S1
the enzyme also methylates uracil1939 in 23S rRNA
?
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae S1
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also methylates uracil1939 in 23S rRNA
734806
Streptococcus pneumoniae
?
-
-
-
-
additional information
the enzyme also methylates uracil1939 in 23S rRNA
734806
Streptococcus pneumoniae S1
?
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
734806
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
734806
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Streptococcus pneumoniae
the enzyme also methylates uracil1939 in 23S rRNA
?
-
-
-
additional information
Streptococcus pneumoniae S1
the enzyme also methylates uracil1939 in 23S rRNA
?
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also methylates uracil1939 in 23S rRNA
734806
Streptococcus pneumoniae
?
-
-
-
-
additional information
the enzyme also methylates uracil1939 in 23S rRNA
734806
Streptococcus pneumoniae S1
?
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
734806
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
734806
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
Streptococcus pneumoniae
metabolism
methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
Streptococcus pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
Streptococcus pneumoniae
metabolism
methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
Streptococcus pneumoniae
Other publictions for EC 2.1.1.189
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734806
Shoji
RlmCD-mediated U747 methylatio ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Nucleic Acids Res.
43
8964-8972
2015
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4
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4
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4
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4
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4
-
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-
-
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-
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-
-
2
2
-
-
-
720592
Desmolaize
A single methyltransferase Yef ...
Bacillus subtilis 168, Bacillus subtilis
Nucleic Acids Res.
39
9368-9375
2011
-
-
1
-
-
-
-
-
-
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4
-
7
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-
-
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-
4
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-
1
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1
1
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4
-
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4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721031
Auxilien
Specificity shifts in the rRNA ...
Escherichia coli, Pyrococcus abyssi
RNA
17
45-53
2011
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-
-
-
-
-
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2
-
10
-
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5
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1
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1
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2
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5
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1
1
-
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705956
Madsen
Identifying the methyltransfer ...
Escherichia coli
Nucleic Acids Res.
31
4738-4746
2003
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1
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-
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-
-
-
-
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1
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-
-
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1
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1
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1
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1
1
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