BRENDA - Enzyme Database show
show all sequences of 2.1.1.188

The tylosin-resistance methyltransferase RlmAII (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748

Douthwaite, S.; Crain, P.F.; Liu, M.; Poehlsgaard, J.; J. Mol. Biol. 337, 1073-1077 (2004)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptomyces fradiae
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces fradiae
Q9S1M6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
659709
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
the methylation site of RlmAII is identified by liquid chromatography/electrospray ionization mass spectrometry as the N-1 position of 23S rRNA nucleotide G748
659709
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptomyces fradiae
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
659709
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
the methylation site of RlmAII is identified by liquid chromatography/electrospray ionization mass spectrometry as the N-1 position of 23S rRNA nucleotide G748
659709
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Other publictions for EC 2.1.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734806
Shoji
RlmCD-mediated U747 methylatio ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Nucleic Acids Res.
43
8964-8972
2015
1
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1
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4
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1
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1
1
1
1
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733110
Takaya
Methylation of 23S rRNA nucleo ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Antimicrob. Agents Chemother.
57
3789-3796
2013
-
-
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9
-
-
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1
2
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5
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2
1
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9
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2
1
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2
2
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699533
Douthwaite
Interaction of the tylosin-res ...
Streptococcus pneumoniae
J. Mol. Biol.
378
969-975
2008
-
-
-
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-
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4
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1
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705115
Lebars
Recognition elements in rRNA f ...
Streptococcus pneumoniae
J. Mol. Biol.
372
525-534
2007
-
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1
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-
-
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-
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3
-
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1
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1
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1
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1
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1
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659709
Douthwaite
The tylosin-resistance methylt ...
Streptomyces fradiae
J. Mol. Biol.
337
1073-1077
2004
-
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658541
Lebars
Structure of 23S rRNA hairpin ...
Streptococcus pneumoniae
EMBO J.
22
183-192
2003
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1
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4
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1
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441501
Liu
Methylation at nucleotide G745 ...
Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Enterococcus faecalis, Lactobacillus plantarum, Lactococcus lactis, Micromonospora griseorubida, Rhodococcus corynebacterioides, Streptococcus pneumoniae, Streptococcus thermophilus, Streptomyces fradiae
Mol. Microbiol.
44
195-204
2002
-
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5
-
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-
-
-
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11
-
17
-
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5
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11
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11
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11
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5
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11
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5
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11
-
11
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11
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705762
Liu
The tylosin resistance gene tl ...
Streptomyces fradiae
Mol. Microbiol.
37
811-820
2000
-
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1
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1
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1
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