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show all sequences of 2.1.1.188

Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria

Liu, M.; Douthwaite, S.; Mol. Microbiol. 44, 195-204 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Bacillus subtilis
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptomyces fradiae
-
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Bacillus megaterium
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Enterococcus faecalis
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Lactobacillus plantarum
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Lactococcus lactis
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Rhodococcus corynebacterioides
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus thermophilus
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Bacillus subtilis
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Corynebacterium glutamicum
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Micromonospora griseorubida
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptomyces fradiae
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Bacillus subtilis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Corynebacterium glutamicum
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Enterococcus faecalis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Lactobacillus plantarum
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Lactococcus lactis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Micromonospora griseorubida
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Rhodococcus corynebacterioides
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptococcus pneumoniae
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptococcus thermophilus
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptomyces fradiae
Q9S1M6
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptococcus pneumoniae
-
Streptomyces fradiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Bacillus megaterium
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Enterococcus faecalis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Lactobacillus plantarum
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Lactococcus lactis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Rhodococcus corynebacterioides
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Streptococcus thermophilus
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Corynebacterium glutamicum
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Micromonospora griseorubida
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus megaterium
37
-
assay at
Bacillus subtilis
37
-
assay at
Corynebacterium glutamicum
37
-
assay at
Enterococcus faecalis
37
-
assay at
Lactobacillus plantarum
37
-
assay at
Lactococcus lactis
37
-
assay at
Micromonospora griseorubida
37
-
assay at
Rhodococcus corynebacterioides
37
-
assay at
Streptococcus pneumoniae
37
-
assay at
Streptococcus thermophilus
37
-
assay at
Streptomyces fradiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Bacillus megaterium
7.5
-
assay at
Bacillus subtilis
7.5
-
assay at
Corynebacterium glutamicum
7.5
-
assay at
Enterococcus faecalis
7.5
-
assay at
Lactobacillus plantarum
7.5
-
assay at
Lactococcus lactis
7.5
-
assay at
Micromonospora griseorubida
7.5
-
assay at
Rhodococcus corynebacterioides
7.5
-
assay at
Streptococcus pneumoniae
7.5
-
assay at
Streptococcus thermophilus
7.5
-
assay at
Streptomyces fradiae
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptomyces fradiae
-
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Bacillus megaterium
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Enterococcus faecalis
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Lactobacillus plantarum
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Lactococcus lactis
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Rhodococcus corynebacterioides
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus thermophilus
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Bacillus subtilis
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Corynebacterium glutamicum
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Micromonospora griseorubida
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptomyces fradiae
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptococcus pneumoniae
-
Streptomyces fradiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Bacillus megaterium
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Enterococcus faecalis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Lactobacillus plantarum
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Lactococcus lactis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Rhodococcus corynebacterioides
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
441501
Streptococcus thermophilus
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Bacillus subtilis
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Corynebacterium glutamicum
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Micromonospora griseorubida
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
441501
Streptomyces fradiae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus megaterium
37
-
assay at
Bacillus subtilis
37
-
assay at
Corynebacterium glutamicum
37
-
assay at
Enterococcus faecalis
37
-
assay at
Lactobacillus plantarum
37
-
assay at
Lactococcus lactis
37
-
assay at
Micromonospora griseorubida
37
-
assay at
Rhodococcus corynebacterioides
37
-
assay at
Streptococcus pneumoniae
37
-
assay at
Streptococcus thermophilus
37
-
assay at
Streptomyces fradiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Bacillus megaterium
7.5
-
assay at
Bacillus subtilis
7.5
-
assay at
Corynebacterium glutamicum
7.5
-
assay at
Enterococcus faecalis
7.5
-
assay at
Lactobacillus plantarum
7.5
-
assay at
Lactococcus lactis
7.5
-
assay at
Micromonospora griseorubida
7.5
-
assay at
Rhodococcus corynebacterioides
7.5
-
assay at
Streptococcus pneumoniae
7.5
-
assay at
Streptococcus thermophilus
7.5
-
assay at
Streptomyces fradiae
Other publictions for EC 2.1.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734806
Shoji
RlmCD-mediated U747 methylatio ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Nucleic Acids Res.
43
8964-8972
2015
1
-
1
-
-
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
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1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
733110
Takaya
Methylation of 23S rRNA nucleo ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Antimicrob. Agents Chemother.
57
3789-3796
2013
-
-
-
-
9
-
-
-
-
-
1
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
699533
Douthwaite
Interaction of the tylosin-res ...
Streptococcus pneumoniae
J. Mol. Biol.
378
969-975
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705115
Lebars
Recognition elements in rRNA f ...
Streptococcus pneumoniae
J. Mol. Biol.
372
525-534
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659709
Douthwaite
The tylosin-resistance methylt ...
Streptomyces fradiae
J. Mol. Biol.
337
1073-1077
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658541
Lebars
Structure of 23S rRNA hairpin ...
Streptococcus pneumoniae
EMBO J.
22
183-192
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441501
Liu
Methylation at nucleotide G745 ...
Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Enterococcus faecalis, Lactobacillus plantarum, Lactococcus lactis, Micromonospora griseorubida, Rhodococcus corynebacterioides, Streptococcus pneumoniae, Streptococcus thermophilus, Streptomyces fradiae
Mol. Microbiol.
44
195-204
2002
-
-
5
-
-
-
-
-
-
-
-
11
-
17
-
-
5
-
-
-
-
-
11
-
11
-
-
-
11
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
5
-
-
-
-
11
-
11
-
-
-
11
-
-
-
-
-
-
-
-
-
705762
Liu
The tylosin resistance gene tl ...
Streptomyces fradiae
Mol. Microbiol.
37
811-820
2000
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
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-
-
-
-
-
-
-
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1
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-
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-
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1
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1
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2
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