BRENDA - Enzyme Database
show all sequences of 2.1.1.188

RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility

Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.; Nucleic Acids Res. 43, 8964-8972 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme
Streptococcus pneumoniae
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptococcus pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
x * 32000, SDS-PAGE
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae S1
-
-
-
Purification (Commentary)
Commentary
Organism
glutathione Sepharose column chromatography and Superdex 75 gel filtration; glutathione Sepharose column chromatography and Superdex75 gel filtration
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
734806
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
734806
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Streptococcus pneumoniae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptococcus pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
x * 32000, SDS-PAGE
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione Sepharose column chromatography and Superdex 75 gel filtration; glutathione Sepharose column chromatography and Superdex75 gel filtration
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
734806
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
734806
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Streptococcus pneumoniae
Expression
Organism
Commentary
Expression
Streptococcus pneumoniae
RlmCD disruption does not influence the level of the enzyme
additional information
General Information
General Information
Commentary
Organism
metabolism
the enzyme enhances binding of telithromycin to A752 in ribosomes
Streptococcus pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme enhances binding of telithromycin to A752 in ribosomes
Streptococcus pneumoniae
Expression (protein specific)
Organism
Commentary
Expression
Streptococcus pneumoniae
RlmCD disruption does not influence the level of the enzyme
additional information
Other publictions for EC 2.1.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734806
Shoji
RlmCD-mediated U747 methylatio ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Nucleic Acids Res.
43
8964-8972
2015
1
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1
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2
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4
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1
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2
1
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1
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1
2
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1
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2
1
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-
-
-
-
1
1
1
1
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-
733110
Takaya
Methylation of 23S rRNA nucleo ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Antimicrob. Agents Chemother.
57
3789-3796
2013
-
-
-
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9
-
-
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1
2
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5
-
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2
1
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9
-
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1
2
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2
1
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-
-
-
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2
2
-
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699533
Douthwaite
Interaction of the tylosin-res ...
Streptococcus pneumoniae
J. Mol. Biol.
378
969-975
2008
-
-
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4
-
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1
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1
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1
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1
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705115
Lebars
Recognition elements in rRNA f ...
Streptococcus pneumoniae
J. Mol. Biol.
372
525-534
2007
-
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1
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-
-
-
-
-
-
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3
-
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1
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1
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1
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1
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1
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659709
Douthwaite
The tylosin-resistance methylt ...
Streptomyces fradiae
J. Mol. Biol.
337
1073-1077
2004
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1
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2
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1
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2
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658541
Lebars
Structure of 23S rRNA hairpin ...
Streptococcus pneumoniae
EMBO J.
22
183-192
2003
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1
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4
-
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1
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1
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1
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441501
Liu
Methylation at nucleotide G745 ...
Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Enterococcus faecalis, Lactobacillus plantarum, Lactococcus lactis, Micromonospora griseorubida, Rhodococcus corynebacterioides, Streptococcus pneumoniae, Streptococcus thermophilus, Streptomyces fradiae
Mol. Microbiol.
44
195-204
2002
-
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5
-
-
-
-
-
-
-
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11
-
17
-
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5
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11
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11
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11
-
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5
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11
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5
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11
-
11
-
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11
-
-
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705762
Liu
The tylosin resistance gene tl ...
Streptomyces fradiae
Mol. Microbiol.
37
811-820
2000
-
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1
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1
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1
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1
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