BRENDA - Enzyme Database
show all sequences of 2.1.1.188

Methylation of 23S rRNA nucleotide G748 by RlmAII methyltransferase renders Streptococcus pneumoniae telithromycin susceptible

Takaya, A.; Sato, Y.; Shoji, T.; Yamamoto, T.; Antimicrob. Agents Chemother. 57, 3789-3796 (2013)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
A135T
inactive
Streptococcus pneumoniae
C23R
inactive
Streptococcus pneumoniae
C36R
inactive
Streptococcus pneumoniae
L113P
inactive
Streptococcus pneumoniae
L164P
inactive
Streptococcus pneumoniae
L165P
inactive
Streptococcus pneumoniae
L247P
inactive
Streptococcus pneumoniae
R200C
inactive
Streptococcus pneumoniae
Y212C
inactive
Streptococcus pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31500
-
x * 31500, calculated from amino acid sequence
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae S1
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
733110
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
733110
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 31500, calculated from amino acid sequence
Streptococcus pneumoniae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A135T
inactive
Streptococcus pneumoniae
C23R
inactive
Streptococcus pneumoniae
C36R
inactive
Streptococcus pneumoniae
L113P
inactive
Streptococcus pneumoniae
L164P
inactive
Streptococcus pneumoniae
L165P
inactive
Streptococcus pneumoniae
L247P
inactive
Streptococcus pneumoniae
R200C
inactive
Streptococcus pneumoniae
Y212C
inactive
Streptococcus pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31500
-
x * 31500, calculated from amino acid sequence
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
733110
Streptococcus pneumoniae
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
733110
Streptococcus pneumoniae S1
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31500, calculated from amino acid sequence
Streptococcus pneumoniae
General Information
General Information
Commentary
Organism
malfunction
the inactivation of the enzyme confers telithromycin resistance
Streptococcus pneumoniae
physiological function
methylation of 23S rRNA nucleotide G748 by RlmAII methyltransferase renders Streptococcus pneumoniae telithromycin susceptible
Streptococcus pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the inactivation of the enzyme confers telithromycin resistance
Streptococcus pneumoniae
physiological function
methylation of 23S rRNA nucleotide G748 by RlmAII methyltransferase renders Streptococcus pneumoniae telithromycin susceptible
Streptococcus pneumoniae
Other publictions for EC 2.1.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734806
Shoji
RlmCD-mediated U747 methylatio ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Nucleic Acids Res.
43
8964-8972
2015
1
-
1
-
-
-
-
-
-
-
1
2
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4
-
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1
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2
1
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1
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1
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1
2
-
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1
-
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-
-
2
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
733110
Takaya
Methylation of 23S rRNA nucleo ...
Streptococcus pneumoniae, Streptococcus pneumoniae S1
Antimicrob. Agents Chemother.
57
3789-3796
2013
-
-
-
-
9
-
-
-
-
-
1
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
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9
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
699533
Douthwaite
Interaction of the tylosin-res ...
Streptococcus pneumoniae
J. Mol. Biol.
378
969-975
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705115
Lebars
Recognition elements in rRNA f ...
Streptococcus pneumoniae
J. Mol. Biol.
372
525-534
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
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-
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-
-
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-
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1
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1
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1
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-
-
-
-
-
-
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-
659709
Douthwaite
The tylosin-resistance methylt ...
Streptomyces fradiae
J. Mol. Biol.
337
1073-1077
2004
-
-
-
-
-
-
-
-
-
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1
-
2
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2
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1
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2
-
-
-
-
-
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658541
Lebars
Structure of 23S rRNA hairpin ...
Streptococcus pneumoniae
EMBO J.
22
183-192
2003
-
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1
-
-
-
-
-
-
-
-
-
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4
-
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1
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1
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1
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1
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1
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-
441501
Liu
Methylation at nucleotide G745 ...
Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Enterococcus faecalis, Lactobacillus plantarum, Lactococcus lactis, Micromonospora griseorubida, Rhodococcus corynebacterioides, Streptococcus pneumoniae, Streptococcus thermophilus, Streptomyces fradiae
Mol. Microbiol.
44
195-204
2002
-
-
5
-
-
-
-
-
-
-
-
11
-
17
-
-
5
-
-
-
-
-
11
-
11
-
-
-
11
-
-
-
-
-
-
-
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5
-
-
-
-
-
-
-
-
-
-
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11
-
-
-
5
-
-
-
-
11
-
11
-
-
-
11
-
-
-
-
-
-
-
-
-
705762
Liu
The tylosin resistance gene tl ...
Streptomyces fradiae
Mol. Microbiol.
37
811-820
2000
-
-
1
-
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-
-
-
-
-
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1
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1
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1
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2
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1
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1
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1
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2
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