Literature summary for 2.1.1.187 extracted from

Hansen, L.H.; Kirpekar, F.; Douthwaite, S.
Recognition of nucleotide G745 in 23 S ribosomal RNA by the RrmA methyltransferase (2001), J. Mol. Biol., 310, 1001-1010.

Search for more literature for 2.1.1.187

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine745 in 23S rRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P36999	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine745 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	methylation of the N1 position of nucleotide G745 in hairpin 35 of Escherichia coli 23S ribosomal RNA. Progressive truncation of the rRNA substrate shows that structures in stem-loops 33, 34 and 35 are required for methylation by RrmA. Multiple contacts between nucleotides in these stem-loops and RrmA are confirmed in footprinting experiments. No other RrmA contact is evident elsewhere in the rRNA. The RrmA contact sites on the rRNA are inaccessible in ribosomal particles and, consistent with this, 50S subunits or 70S ribosomes are not substrates for RrmA methylation. Methylate their target nucleotides only in the free RNA	Escherichia coli	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA	-	?

General Information

General Information	Comment	Organism
malfunction	lack of G745 methylation results in reduced rates of protein synthesis and growth. Addition of recombinant plasmid-encoded rrmA to an rrmA-defficient strain remedies these defects	Escherichia coli

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Release 2023.1 (January 2023)