Literature summary for 2.1.1.186 extracted from

Guo, H.Y.; Gao, Z.Q.; Zhang, H.; Wei, Y.; Xu, J.H.; Wang, W.Y.; Yan, A.X.; Dong, Y.H.
Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 640-642.

Search for more literature for 2.1.1.186

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) Star cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 16% (w/v) polyethylene glycol 3350, 0.05 M citric acid, 0.05 M bis-tris propane pH 5.0	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0ADR6	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-nitrilotriacetic acid resin column chromatography, heparin column chromatography, and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
Cm2498	-	Escherichia coli
RlmM	-	Escherichia coli

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Release 2023.1 (January 2023)