Cloned (Comment) | Organism |
---|---|
expressed to a high level in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000344 | - |
623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | pH 7.5, 37°C, a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | Bacillus subtilis | |
0.0000375 | - |
23S rRNA | pH 7.5, 37°C, a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | Bacillus subtilis | |
0.000144 | - |
623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA with mutation A2086T | pH 7.5, 37°C, a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | Bacillus subtilis | |
0.00091 | - |
262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | pH 7.5, 37°C, a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | Bacillus subtilis | ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalyzing the methylation of 23S rRNA at a specific adenine residue (A2085 in Bacillus subtilis) | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P13956 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA = 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | a model of RNA-ErmC interaction involving multiple binding sites is proposed from the kinetic data | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalyzing the methylation of 23S rRNA at a specific adenine residue (A2085 in Bacillus subtilis) | Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | the specificity of methylation on adenine2085 is confirmed by site-directed mutagenesis. All three mutated domain V fragments, A2085T, A2085G, and A2085C, are methylated to less than 10% of the level observed with the correct domain V RNA fragment. The G2084A change reduces the methylation of the resultant domain V fragment to ca. 12% of the level of the wild-type domain V fragment | Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2085 in a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | a 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA can be utilized efficiently as a substrate for methylation at adenine2085 | Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2085 in a 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | - |
Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in a 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2085 in a 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA with mutation A2086T | the A2086T change is methylated to ca. 50% of the level of wild-type domain V | Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in a 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA with mutation A2086T | - |
? | |
S-adenosyl-L-methionine + 23S rRNA | - |
Bacillus subtilis | S-adenosyl-L-homocysteine + 23S rRNA containing N6-dimethyladenine | - |
? | |
S-adenosyl-L-methionine + 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | - |
Bacillus subtilis | S-adenosyl-L-homocysteine + 262-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA containing N6-dimethyladenine | - |
? | |
S-adenosyl-L-methionine + 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA | - |
Bacillus subtilis | S-adenosyl-L-homocysteine + 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA containing N6-dimethyladenine | - |
? | |
S-adenosyl-L-methionine + 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA with mutation A2086T | - |
Bacillus subtilis | S-adenosyl-L-homocysteine + 623-nucleotide RNA fragment within domain V of Bacillus subtilis 23S rRNA with mutation A2086T containing N6-dimethyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ErmC' methyltransferase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalyzing the methylation of 23S rRNA at a specific adenine residue (A2085 in Bacillus subtilis) | Bacillus subtilis |