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Literature summary for 2.1.1.184 extracted from

  • Bussiere, D.E.; Muchmore, S.W.; Dealwis, C.G.; Schluckebier, G.; Nienaber, V.L.; Edalji, R.P.; Walter, K.A.; Ladror, U.S.; Holzman, T.F.; Abad-Zapatero, C.
    Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria (1998), Biochemistry, 37, 7103-7112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the expression of ErmC' by translational coupling to kdsB, under the control of the T7lac promoter. The pTERM31 plasmid is transformed into Escherichia coli strain BL219(DE3)/pLysS Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of ErmC' are obtained by the hanging-drop vapor diffusion method. Crystal structure of ErmC' (a naturally occurring variant of ErmC) determined at 3.0 A resolution by multiple anomalous diffraction phasing methods. The structure consists of a conserved alpha/beta amino-terminal domain which binds the cofactor S-adenosyl-L-methionine, followed by a smaller, alpha-helical RNA-recognition domain Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P13956
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-

Purification (Commentary)

Purification (Comment) Organism
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Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA
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Bacillus subtilis 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA
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?

Synonyms

Synonyms Comment Organism
ErmC' a naturally occuring variant of ErmC Bacillus subtilis